Detailed information of TA system TA140564

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2106830..2107661
• Replicon: chromosome
• Accession: NZ_CP044311
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: F7D06_RS10695
• Protein ID: WP_000854814.1
• Coordinates: 2107287..2107661 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H0EA76
• Locus tag: F7D06_RS10690
• Protein ID: WP_001285592.1
• Coordinates: 2106830..2107198 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS10660	2103734..2104189	+	456	WP_000581504.1	hypothetical protein	-
F7D06_RS10665	2104268..2104501	+	234	WP_001119724.1	DUF905 family protein	-
F7D06_RS10670	2104601..2105419	+	819	WP_047090156.1	DUF945 domain-containing protein	-
F7D06_RS10675	2105501..2105980	+	480	WP_000860086.1	antirestriction protein	-
F7D06_RS10680	2105996..2106472	+	477	WP_001186726.1	RadC family protein	-
F7D06_RS10685	2106535..2106756	+	222	WP_000692323.1	DUF987 domain-containing protein	-
F7D06_RS10690	2106830..2107198	+	369	WP_001285592.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
F7D06_RS10695	2107287..2107661	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
F7D06_RS10700	2107658..2107852	+	195	WP_000988600.1	hypothetical protein	-
F7D06_RS10705	2107865..2107978	+	114	WP_001161660.1	DUF957 domain-containing protein	-
F7D06_RS10710	2108267..2108395	-	129	Protein_2092	transposase domain-containing protein	-
F7D06_RS10715	2108467..2108649	+	183	WP_001016348.1	hypothetical protein	-
F7D06_RS10720	2108750..2109079	-	330	WP_000450409.1	DUF496 family protein	-
F7D06_RS10725	2109251..2110309	-	1059	WP_001200891.1	FUSC family protein	-
F7D06_RS10730	2110507..2110980	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
F7D06_RS10735	2111099..2112265	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T140564 WP_000854814.1 NZ_CP044311:2107287-2107661 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T140564 NZ_CP059301:155522-155812 [Acinetobacter baumannii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCGCTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGAAAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGGATTGGGCGTGAAGCACTCTACAAAGCCCTACGTCAGGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAGCTCACAGTACAACACGCTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13636.51 Da
• Isoelectric Point: 6.6249

>AT140564 WP_001285592.1 NZ_CP044311:2106830-2107198 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT140564 NZ_CP059301:155224-155520 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTTCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATCCGCCTGAA
TCGTCGATTAGATAAGGTTCAACGTGGAAACTGGGGAGACATTAAACCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTTCTGGATGGAGGATGTATTACATCCAACATGGTGACGTTGTGATTGTAATGCTTGGTGGTGGAGATAAATCA
ACTCAACAACAAGACATCGATCGGGCAGTCAAACTATCGAAAACATTGGAGGATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A0H0EA76