Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 266027..266645 Replicon chromosome
Accession NZ_CP044311
Organism Escherichia coli strain RM13752

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F7D06_RS01255 Protein ID WP_001291435.1
Coordinates 266027..266245 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag F7D06_RS01260 Protein ID WP_000344800.1
Coordinates 266271..266645 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D06_RS01220 261316..261888 + 573 WP_000779831.1 YbaY family lipoprotein -
F7D06_RS01225 261919..262230 - 312 WP_000409911.1 MGMT family protein -
F7D06_RS01235 262609..262962 + 354 WP_000878140.1 DUF1428 domain-containing protein -
F7D06_RS01240 263004..264554 - 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F7D06_RS01245 264718..265188 - 471 WP_000136192.1 YlaC family protein -
F7D06_RS01250 265304..265855 - 552 WP_001727569.1 maltose O-acetyltransferase -
F7D06_RS01255 266027..266245 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
F7D06_RS01260 266271..266645 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
F7D06_RS01265 267191..270340 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
F7D06_RS01270 270363..271556 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140550 WP_001291435.1 NZ_CP044311:c266245-266027 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140550 NZ_CP059298:256585-256887 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATTAAAAATCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAACAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTTCTTTTTGGTGAAGTTGAGGGGGAACTATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT140550 WP_000344800.1 NZ_CP044311:c266645-266271 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140550 NZ_CP059298:256884-257168 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References