Detailed information of TA system TA140549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 231729..232566
• Replicon: chromosome
• Accession: NZ_CP044311
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: F7D06_RS01085
• Protein ID: WP_000227784.1
• Coordinates: 231729..232271 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: F7D06_RS01090
• Protein ID: WP_050938765.1
• Coordinates: 232255..232566 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS01065	227268..228179	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
F7D06_RS01070	228347..228838	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
F7D06_RS01075	228966..230330	-	1365	WP_001000978.1	MFS transporter	-
F7D06_RS01080	230738..231673	+	936	WP_001297127.1	sel1 repeat family protein	-
F7D06_RS01085	231729..232271	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
F7D06_RS01090	232255..232566	-	312	WP_050938765.1	DUF1778 domain-containing protein	Antitoxin
F7D06_RS01095	232751..233641	-	891	WP_000971336.1	heme o synthase	-
F7D06_RS01100	233653..233982	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
F7D06_RS01105	233982..234596	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
F7D06_RS01110	234586..236577	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
F7D06_RS01115	236599..237546	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T140549 WP_000227784.1 NZ_CP044311:c232271-231729 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T140549 NZ_CP059297:2646492-2646680 [Brevundimonas sp. AJA228-03]
ATGAACAGCCGCGATGTCATCAAGGCCTTGCACGCCGATGGATGGATCGAGGTTGCGCATAAGGGATCGCACAAGCAGTT
CCGCCACCCGATCAAGCCCGGTCGCGTGACGGTGCCGCATCCCAAACGCGACTTTCCGATCGCCACCCTGCGCTCGATCG
AGCGTCAGGCACAGATCGTCCTGAGGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11357.05 Da
• Isoelectric Point: 10.6798

>AT140549 WP_050938765.1 NZ_CP044311:c232566-232255 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLNNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT140549 NZ_CP059297:2646685-2647077 [Brevundimonas sp. AJA228-03]
ATGGCCCAGTTCATCGCCCTGATCCACAAGGATGCCGACAGCGACTACGGCGTCCACTTCCCCGATCTGCCGGGCCTGGC
CACCGCCGGGACGACGCTGGACGAGGCGCGCGCCTTTGCCGAAGAGGCTCTGGTCCTGCATCTCGAAGGTCTGGCCGAGG
ATGGCGAACCCATTCCCGAAGCCAGCAGCCTTGAGACCATCATGAATGATCCCGGGAACCGGGACGGGGTGGCCATCCTC
GTTCGGGCCGAGACGCGGTCGCGCGCTGTCCGGGTCAACATCACACTGCCGGAAGATGCCCTTCGCGACATCGACACCTA
TGCCGAGGCCCATGGGTTCACCCGCTCGGGCTTTCTGGCCCAGGCGGCCAGAAAGCTGATGCAGTCGGCCTGA

Structures

Toxin

• PDB: 7BYY