Detailed information of TA system TA140548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1472452..1473057
• Replicon: chromosome
• Accession: NZ_CP044310
• Organism: Paenibacillus xylanilyticus strain W4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4B4V0
• Locus tag: F4V51_RS06475
• Protein ID: WP_024630714.1
• Coordinates: 1472707..1473057 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: F4V51_RS06470
• Protein ID: WP_170948137.1
• Coordinates: 1472452..1472703 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V51_RS06455	1467517..1469031	-	1515	WP_153977344.1	serine hydrolase	-
F4V51_RS06460	1469321..1470532	+	1212	WP_153977345.1	outer membrane lipoprotein-sorting protein	-
F4V51_RS06465	1470972..1472162	+	1191	WP_153977346.1	alanine racemase	-
F4V51_RS06470	1472452..1472703	+	252	WP_170948137.1	ribbon-helix-helix protein, CopG family	Antitoxin
F4V51_RS06475	1472707..1473057	+	351	WP_024630714.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F4V51_RS06480	1473290..1475479	+	2190	WP_153977347.1	alpha-galactosidase	-
F4V51_RS06485	1475778..1477976	+	2199	WP_201281206.1	RNA-binding transcriptional accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12778.77 Da
• Isoelectric Point: 5.1184

>T140548 WP_024630714.1 NZ_CP044310:1472707-1473057 [Paenibacillus xylanilyticus]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTCIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKLVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T140548 NZ_CP059297:c2643806-2643552 [Brevundimonas sp. AJA228-03]
ATGAACAGCAAGCATACGAAGACGCTGTCGGCGGTATTCGCCGATCCGGTGTCCGGAACGATCGAATGGGCTGCGATCGA
AAATCTGCTGGTCGCGGTTGGGTGCCACATCGTCGAGGGGGCGGGCTCAAGGGTCCGGTTTGTGCGTGGTGTCGAAGCCG
AGTTCTTTCACAGGCCTCATCCCGCCAGGGAGGCCAAGCGGTATCAGGTCAGGGCGGCGCGGGCCTTTCTGATCAGGCTG
GGAGTAGAGCCATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9558.96 Da
• Isoelectric Point: 5.1255

>AT140548 WP_170948137.1 NZ_CP044310:1472452-1472703 [Paenibacillus xylanilyticus]
MISLPDHLLQEVDGIVALENSNRSELIRQAMKLYLTERKKRYIRESMQRGYMEMAKINLTMASEAFHAEEDADSTLDRLV
SGV

Nucleotide

• Length: 252 bp

>AT140548 NZ_CP059297:c2643555-2643199 [Brevundimonas sp. AJA228-03]
ATGAGCATCATGACATACAAAGGGTATTCGGCGGCGGTCGAATTCGATGCCGACGACGAGATTTTCTTCGGCAAACTGGC
CGGAATTCGGGACGGCGTCGGCTTTCATGCGGATACGGCGCGCGAGTTGAAGGCGGCCTTCCGGGAGGCGGTCGATGACT
ACATCGAGACCTGCGCCAGGATCGGGAAGTCGCCGGACAAACCTTACTCTGGCAAGGTGATGTTTCGGGTCGATCCCGAA
GTTCATGCGCGCGTCACAAAGGCGGCAGAGCTGTCGGGGCGGAGTCTGAACGCCTGGGCCGAGGACGCGCTGAGGCGGGC
GGCGGATGCGGACATGGCCAAGGCGGTGGCGGTCTGA

Structures

Toxin

• AlphaFold DB: W4B4V0