Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4479748..4480386 Replicon chromosome
Accession NZ_CP044293
Organism Escherichia coli strain P276M

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag F6P95_RS21670 Protein ID WP_000813794.1
Coordinates 4479748..4479924 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag F6P95_RS21675 Protein ID WP_001270286.1
Coordinates 4479970..4480386 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6P95_RS21650 4475367..4476581 - 1215 WP_071597387.1 BenE family transporter YdcO -
F6P95_RS21655 4476634..4477170 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
F6P95_RS21660 4477243..4479204 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
F6P95_RS21665 4479296..4479526 - 231 WP_000494244.1 YncJ family protein -
F6P95_RS21670 4479748..4479924 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
F6P95_RS21675 4479970..4480386 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
F6P95_RS21680 4480465..4481871 + 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
F6P95_RS21685 4482116..4483261 + 1146 WP_072778914.1 ABC transporter substrate-binding protein -
F6P95_RS21690 4483279..4484292 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
F6P95_RS21695 4484293..4485234 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4474062..4475327 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T140547 WP_000813794.1 NZ_CP044293:4479748-4479924 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T140547 NZ_CP059297:c2599825-2599415 [Brevundimonas sp. AJA228-03]
ATGTCACTGTTCATGGTCGATACCAGCGTCTGTATCCGGGCGCTGAGAGGGCGTTCACGCCTATTGATGGATCGCTTTCA
GGAAGAAGCAGACAGGATCGCCATCTCCTCGGTGGTCTTGATGGAACTGCTGTATGGCGCGGCGGTTTCGGCCCGCCCCC
GGCATCACAGGTCCGAGGTTGAAGACTTCGTCGATCGCCTGATGGTCCTGGACCTCGATTCGGAGGCTGCCGCCCACGCT
GCCGACATCAAGGCGGATCTGCGCGCCAAAGGAACGCCGATCGGCGTCCACGACACCCTGATCGCCGGACATGCCAGAAG
TCGGAGTTCGATCCTGATCACGGGCAATCTCAACGAGTTCAAGCGGGTCGATGGCCTGCGGTGCGACGACTGGCCGGACA
CACAGACATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT140547 WP_001270286.1 NZ_CP044293:4479970-4480386 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT140547 NZ_CP059297:c2600058-2599825 [Brevundimonas sp. AJA228-03]
ATGACCCTGCCTCGCGCCACGGTATTCAGAACCAACCGCTCCCAGGCGATCCGCATCCCGAAAGCGGTGGCCTTTCCCGA
TGACGTCAAGGAGTTGGTCGCCATTCGGGAAGGGCGGGGACTGCTGCTTGTTCCGCCGGATGATGTCTGGACGAGCTTCT
TCAACAGGCCGGGCATCGATATCGAAGAGCCGGAAGACCCGATCGACGATTCACCGCCGGTCAGCTTCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References