Detailed information of TA system TA140522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 15063..16636
• Replicon: chromosome
• Accession: NZ_CP044293
• Organism: Escherichia coli strain P276M

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: F6P95_RS00075
• Protein ID: WP_072778584.1
• Coordinates: 15063..16370 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: F6P95_RS00080
• Protein ID: WP_001301023.1
• Coordinates: 16370..16636 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6P95_RS00050	10111..10641	-	531	WP_000876763.1	type 1 fimbrial protein	-
F6P95_RS00055	10655..11842	-	1188	Protein_10	fimbrial biogenesis outer membrane usher protein	-
F6P95_RS00060	11857..12548	-	692	Protein_11	IS1 family transposase	-
F6P95_RS00065	12604..13419	-	816	Protein_12	site-specific integrase	-
F6P95_RS00070	13406..14800	-	1395	WP_171821334.1	site-specific integrase	-
F6P95_RS00075	15063..16370	-	1308	WP_072778584.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
F6P95_RS00080	16370..16636	-	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	12273..12548	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 48711.22 Da
• Isoelectric Point: 8.2543

>T140522 WP_072778584.1 NZ_CP044293:c16370-15063 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSR

Nucleotide

• Length: 1308 bp

>T140522 NZ_CP059288:c3932198-3931911 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT140522 WP_001301023.1 NZ_CP044293:c16636-16370 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT140522 NZ_CP059288:c3931924-3931622 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA