Detailed information of TA system TA140521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 418143..418708
• Replicon: plasmid pRhX5
• Accession: NZ_CP044283
• Organism: Rhodococcus erythropolis strain X5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C3JNM9
• Locus tag: F6X56_RS01995
• Protein ID: WP_003943396.1
• Coordinates: 418352..418708 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: F6X56_RS01990
• Protein ID: WP_151097304.1
• Coordinates: 418143..418355 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6X56_RS01970	413867..414661	+	795	WP_151097298.1	hypothetical protein	-
F6X56_RS01975	414977..416011	-	1035	WP_151097300.1	diiron oxygenase	-
F6X56_RS01980	416200..417447	-	1248	WP_030538129.1	IS256 family transposase	-
F6X56_RS01990	418143..418355	+	213	WP_151097304.1	ribbon-helix-helix domain-containing protein	Antitoxin
F6X56_RS01995	418352..418708	+	357	WP_003943396.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6X56_RS02000	418906..419295	-	390	WP_007728194.1	helix-turn-helix domain-containing protein	-
F6X56_RS32520	420651..420860	+	210	WP_191908632.1	hypothetical protein	-
F6X56_RS02010	421106..421311	-	206	Protein_396	IS110 family transposase	-
F6X56_RS02020	423170..423610	-	441	WP_024488231.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..526979	526979
	-	inside	IScluster/Tn	-	-	416200..422710	6510

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-117)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12844.58 Da
• Isoelectric Point: 6.8817

>T140521 WP_003943396.1 NZ_CP044283:418352-418708 [Rhodococcus erythropolis]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Nucleotide

• Length: 357 bp

>T140521 NZ_CP059288:c3609820-3609422 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7668.40 Da
• Isoelectric Point: 4.1824

>AT140521 WP_151097304.1 NZ_CP044283:418143-418355 [Rhodococcus erythropolis]
MTTKFTISLPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVSADTE

Nucleotide

• Length: 213 bp

>AT140521 NZ_CP059288:c3610116-3609823 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A1F2PUJ5