Detailed information of TA system TA140520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 397672..398207
• Replicon: plasmid pRhX5
• Accession: NZ_CP044283
• Organism: Rhodococcus erythropolis strain X5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A937CJQ5
• Locus tag: F6X56_RS01900
• Protein ID: WP_019749527.1
• Coordinates: 397672..397971 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: F6X56_RS01905
• Protein ID: WP_151097285.1
• Coordinates: 397971..398207 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6X56_RS01885	393797..394867	+	1071	WP_003941261.1	DUF1016 family protein	-
F6X56_RS01890	394996..395793	-	798	WP_151097000.1	hypothetical protein	-
F6X56_RS01895	395793..397643	-	1851	WP_151097283.1	hypothetical protein	-
F6X56_RS01900	397672..397971	-	300	WP_019749527.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6X56_RS01905	397971..398207	-	237	WP_151097285.1	antitoxin	Antitoxin
F6X56_RS01910	398637..399317	-	681	WP_151097287.1	TetR/AcrR family transcriptional regulator	-
F6X56_RS01915	399373..400347	-	975	WP_151097289.1	DUF2637 domain-containing protein	-
F6X56_RS01920	400765..402012	-	1248	WP_030538129.1	IS256 family transposase	-
F6X56_RS01925	402401..402715	-	315	WP_030537937.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..526979	526979
	-	flank	IS/Tn	-	-	400765..402012	1247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10414.98 Da
• Isoelectric Point: 4.6942

>T140520 WP_019749527.1 NZ_CP044283:c397971-397672 [Rhodococcus erythropolis]
MRPIHEANLDSVRPVLVLTREMVRPHLGTVTVAPITGEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNIIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T140520 NZ_CP059288:3393119-3393337 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8757.81 Da
• Isoelectric Point: 6.7266

>AT140520 WP_151097285.1 NZ_CP044283:c398207-397971 [Rhodococcus erythropolis]
MSTQIAVRLPDEIVEFLDDEVREHRAASRASLVLRALERERRRQIAARDAEILSAATSEHDLSPLAHRAVNRQPTGST

Nucleotide

• Length: 237 bp

>AT140520 NZ_CP059288:3392719-3393093 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG