Detailed information of TA system TA140518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 35112..36249
• Replicon: plasmid pHVH-V2937-2
• Accession: NZ_CP044276
• Organism: Enterococcus faecium strain V2937

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: F6440_RS15830
• Protein ID: WP_074394525.1
• Coordinates: 35386..36249 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: F6440_RS15825
• Protein ID: WP_002331065.1
• Coordinates: 35112..35384 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6440_RS15785	30411..31319	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
F6440_RS15790	31316..31858	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
F6440_RS15795	31951..32745	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
F6440_RS15805	33021..33593	+	573	WP_001079938.1	HTH domain-containing protein	-
F6440_RS15815	33891..34787	+	897	WP_002334978.1	ParA family protein	-
F6440_RS15820	34886..35095	+	210	WP_000527318.1	peptide-binding protein	-
F6440_RS15825	35112..35384	+	273	WP_002331065.1	antitoxin	Antitoxin
F6440_RS15830	35386..36249	+	864	WP_074394525.1	zeta toxin family protein	Toxin
F6440_RS15835	36512..37249	-	738	WP_002321849.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
F6440_RS15840	37374..37457	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
F6440_RS15845	37604..38284	+	681	WP_001067799.1	IS6-like element IS1216 family transposase	-
F6440_RS15850	38288..38905	+	618	WP_099124473.1	FtsX-like permease family protein	-
F6440_RS15855	39306..39911	+	606	WP_000599739.1	cell filamentation protein	-
F6440_RS15860	39927..40499	+	573	WP_000170424.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / ant(6)-Ia / aph(3')-III / erm(B)	-	1..40955	40955
	-	inside	IS/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	30411..38284	7873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32611.17 Da
• Isoelectric Point: 6.6627

>T140518 WP_074394525.1 NZ_CP044276:35386-36249 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T140518 NZ_CP059288:2610730-2610837 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT140518 WP_002331065.1 NZ_CP044276:35112-35384 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT140518 NZ_CP059288:c2610683-2610617 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55