Detailed information of TA system TA140517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 28555..29663
• Replicon: plasmid pHVH-V2937-2
• Accession: NZ_CP044276
• Organism: Enterococcus faecium strain V2937

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: F6440_RS15775
• Protein ID: WP_060763469.1
• Coordinates: 28794..29663 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: F6440_RS15770
• Protein ID: WP_000205227.1
• Coordinates: 28555..28779 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6440_RS16285	24145..24315	+	171	WP_000713595.1	hypothetical protein	-
F6440_RS15745	24329..24946	+	618	WP_001062587.1	recombinase family protein	-
F6440_RS15750	24946..25623	+	678	Protein_28	DNA topoisomerase	-
F6440_RS15755	26135..26431	+	297	WP_002360685.1	hypothetical protein	-
F6440_RS15760	26432..26848	+	417	WP_000323438.1	recombinase	-
F6440_RS15765	26850..28412	+	1563	WP_000136908.1	recombinase family protein	-
F6440_RS15770	28555..28779	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
F6440_RS15775	28794..29663	+	870	WP_060763469.1	nucleotidyltransferase domain-containing protein	Toxin
F6440_RS15780	29644..30378	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
F6440_RS15785	30411..31319	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
F6440_RS15790	31316..31858	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
F6440_RS15795	31951..32745	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
F6440_RS15805	33021..33593	+	573	WP_001079938.1	HTH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / ant(6)-Ia / aph(3')-III / erm(B)	-	1..40955	40955
	-	inside	IS/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	30411..38284	7873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32777.35 Da
• Isoelectric Point: 4.7269

>T140517 WP_060763469.1 NZ_CP044276:28794-29663 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T140517 NZ_CP059288:c2400297-2400121 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT140517 WP_000205227.1 NZ_CP044276:28555-28779 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT140517 NZ_CP059288:c2400075-2399659 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA