Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 494196..494767 Replicon chromosome
Accession NZ_CP044274
Organism Enterococcus faecium strain V2937

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag F6440_RS02445 Protein ID WP_002286801.1
Coordinates 494426..494767 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag F6440_RS02440 Protein ID WP_002323011.1
Coordinates 494196..494426 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6440_RS02400 489329..490660 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
F6440_RS02405 490682..491308 + 627 WP_002286816.1 cysteine hydrolase -
F6440_RS02410 491491..492072 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
F6440_RS02420 492804..493151 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
F6440_RS02425 493127..493366 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
F6440_RS02430 493571..493909 - 339 WP_002286804.1 hypothetical protein -
F6440_RS02440 494196..494426 + 231 WP_002323011.1 hypothetical protein Antitoxin
F6440_RS02445 494426..494767 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F6440_RS02450 495617..495802 + 186 WP_002304207.1 hypothetical protein -
F6440_RS02455 496307..496501 + 195 WP_002297028.1 hypothetical protein -
F6440_RS02460 496659..496941 + 283 Protein_474 transposase -
F6440_RS02470 497185..498015 - 831 WP_002286789.1 manganese catalase family protein -
F6440_RS02475 498223..499557 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T140515 WP_002286801.1 NZ_CP044274:494426-494767 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T140515 NZ_CP059288:1747417-1747671 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT140515 WP_002323011.1 NZ_CP044274:494196-494426 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT140515 NZ_CP059288:1747169-1747420 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References