Detailed information of TA system TA140513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 144556..145163
• Replicon: plasmid pHVH-V1836-2
• Accession: NZ_CP044266
• Organism: Enterococcus faecium strain V1836

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: F6439_RS16360
• Protein ID: WP_002321032.1
• Coordinates: 144813..145163 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: F6439_RS16355
• Protein ID: WP_002287514.1
• Coordinates: 144556..144819 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6439_RS16315	139797..140150	-	354	WP_002287659.1	IS66 family insertion sequence element accessory protein TnpB	-
F6439_RS16320	140140..140334	-	195	WP_002285758.1	hypothetical protein	-
F6439_RS16325	140402..140635	-	234	Protein_173	hypothetical protein	-
F6439_RS16330	140878..141537	+	660	WP_002368120.1	hypothetical protein	-
F6439_RS16335	141797..142141	-	345	WP_002368121.1	hypothetical protein	-
F6439_RS16340	142356..142550	+	195	WP_002368123.1	hypothetical protein	-
F6439_RS16345	142629..143420	+	792	Protein_177	glycosyltransferase family 2 protein	-
F6439_RS16350	143520..144200	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
F6439_RS16880	144238..144402	-	165	WP_164450253.1	hypothetical protein	-
F6439_RS16355	144556..144819	+	264	WP_002287514.1	hypothetical protein	Antitoxin
F6439_RS16360	144813..145163	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6439_RS16365	145187..145801	-	615	WP_002318230.1	recombinase family protein	-
F6439_RS16370	146115..146537	+	423	WP_002288787.1	hypothetical protein	-
F6439_RS16375	146547..146750	+	204	WP_002299573.1	hypothetical protein	-
F6439_RS16380	146961..147545	+	585	WP_002299575.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / aac(6')-aph(2'')	-	1..147595	147595
	-	inside	IScluster/Tn	-	-	143520..147545	4025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T140513 WP_002321032.1 NZ_CP044266:144813-145163 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T140513 NZ_CP059288:971734-972069 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT140513 WP_002287514.1 NZ_CP044266:144556-144819 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT140513 NZ_CP059288:971486-971734 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0