Detailed information of TA system TA140512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 51269..51788
• Replicon: plasmid pHVH-V1836-2
• Accession: NZ_CP044266
• Organism: Enterococcus faecium strain V1836

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: F6439_RS15765
• Protein ID: WP_000588503.1
• Coordinates: 51269..51526 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: F6439_RS15770
• Protein ID: WP_000388479.1
• Coordinates: 51519..51788 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6439_RS15725	46519..46794	+	276	WP_001196543.1	hypothetical protein	-
F6439_RS15730	46987..47241	+	255	WP_000199136.1	transposase	-
F6439_RS16860	47382..47606	+	225	WP_158183422.1	IS3 family transposase	-
F6439_RS15740	47647..48809	+	1163	WP_086953888.1	IS3 family transposase	-
F6439_RS15745	48910..49482	+	573	WP_073120187.1	IS3 family transposase	-
F6439_RS15755	49955..50527	-	573	WP_000170424.1	recombinase family protein	-
F6439_RS15760	50543..51148	-	606	WP_000599739.1	cell filamentation protein	-
F6439_RS15765	51269..51526	-	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
F6439_RS15770	51519..51788	-	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
F6439_RS15775	51919..52098	-	180	WP_001120991.1	hypothetical protein	-
F6439_RS15785	52479..53045	-	567	Protein_66	hypothetical protein	-
F6439_RS15790	53121..53801	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
F6439_RS15795	54527..54622	+	96	WP_106913803.1	type I toxin-antitoxin system Fst family toxin	-
F6439_RS15800	54924..55169	-	246	WP_002311817.1	hypothetical protein	-
F6439_RS15805	55514..55798	+	285	WP_000353844.1	hypothetical protein	-
F6439_RS15810	55801..56217	+	417	WP_001232859.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / aac(6')-aph(2'')	-	1..147595	147595
	-	inside	IScluster/Tn	-	-	37924..57817	19893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T140512 WP_000588503.1 NZ_CP044266:c51526-51269 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T140512 NZ_CP059288:881076-881483 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT140512 WP_000388479.1 NZ_CP044266:c51788-51519 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT140512 NZ_CP059288:880829-881095 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7