Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2530067..2530638 Replicon chromosome
Accession NZ_CP044264
Organism Enterococcus faecium strain V1836

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag F6439_RS12650 Protein ID WP_002286801.1
Coordinates 2530067..2530408 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag F6439_RS12655 Protein ID WP_002323011.1
Coordinates 2530408..2530638 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6439_RS12620 2525277..2526611 - 1335 WP_002286788.1 ABC transporter substrate-binding protein -
F6439_RS12625 2526819..2527649 + 831 WP_002286789.1 manganese catalase family protein -
F6439_RS12635 2527893..2528175 - 283 Protein_2380 transposase -
F6439_RS12640 2528333..2528527 - 195 WP_002297028.1 hypothetical protein -
F6439_RS12645 2529032..2529217 - 186 WP_002304207.1 hypothetical protein -
F6439_RS12650 2530067..2530408 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F6439_RS12655 2530408..2530638 - 231 WP_002323011.1 hypothetical protein Antitoxin
F6439_RS12665 2530925..2531263 + 339 WP_002286804.1 hypothetical protein -
F6439_RS12670 2531468..2531707 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
F6439_RS12675 2531683..2532030 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
F6439_RS12685 2532762..2533343 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
F6439_RS12690 2533526..2534152 - 627 WP_002286816.1 cysteine hydrolase -
F6439_RS12695 2534174..2535505 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T140509 WP_002286801.1 NZ_CP044264:c2530408-2530067 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T140509 NZ_CP059288:627804-628118 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT140509 WP_002323011.1 NZ_CP044264:c2530638-2530408 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT140509 NZ_CP059288:628115-628531 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References