140504

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 130114..130757 Replicon plasmid pKPC-2-KP65
Accession NZ_CP044259
Organism Klebsiella pneumoniae strain KP65

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag F6W81_RS28190 Protein ID WP_001044770.1
Coordinates 130341..130757 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag F6W81_RS28185 Protein ID WP_001261282.1
Coordinates 130114..130344 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W81_RS28150 125120..125848 + 729 WP_008324185.1 plasmid SOS inhibition protein A -
F6W81_RS28155 125845..126168 + 324 WP_008324183.1 hypothetical protein -
F6W81_RS28160 126230..126589 + 360 WP_015493069.1 hypothetical protein -
F6W81_RS28165 127215..127589 + 375 WP_008324180.1 hypothetical protein -
F6W81_RS28170 127715..128419 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
F6W81_RS28175 128591..129535 - 945 WP_011977810.1 hypothetical protein -
F6W81_RS28180 129696..130157 - 462 WP_014343465.1 hypothetical protein -
F6W81_RS28185 130114..130344 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F6W81_RS28190 130341..130757 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
F6W81_RS28195 130831..132393 + 1563 WP_004206609.1 AAA family ATPase -
F6W81_RS28200 132378..133400 + 1023 WP_000361404.1 helicase UvrD -
F6W81_RS28205 133656..134353 + 698 Protein_184 IS1 family transposase -
F6W81_RS28210 134382..134654 + 273 Protein_185 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 - 1..144422 144422
- flank IS/Tn - - 127715..128419 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T140504 WP_001044770.1 NZ_CP044259:130341-130757 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T140504 NZ_CP059285:c38629-38423 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT140504 WP_001261282.1 NZ_CP044259:130114-130344 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT140504 NZ_CP059285:38616-38677 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References