Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5358891..5359516 Replicon chromosome
Accession NZ_CP044258
Organism Klebsiella pneumoniae strain KP65

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag F6W81_RS26720 Protein ID WP_002882817.1
Coordinates 5358891..5359274 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag F6W81_RS26725 Protein ID WP_004150355.1
Coordinates 5359274..5359516 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W81_RS26705 5356257..5357159 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
F6W81_RS26710 5357156..5357791 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
F6W81_RS26715 5357788..5358717 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
F6W81_RS26720 5358891..5359274 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
F6W81_RS26725 5359274..5359516 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
F6W81_RS26730 5359721..5360638 + 918 WP_002882812.1 alpha/beta hydrolase -
F6W81_RS26735 5360652..5361593 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
F6W81_RS26740 5361638..5362075 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
F6W81_RS26745 5362072..5362932 - 861 WP_002882807.1 virulence factor BrkB family protein -
F6W81_RS26750 5362926..5363525 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T140499 WP_002882817.1 NZ_CP044258:c5359274-5358891 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T140499 NZ_CP059283:c2788242-2787865 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT140499 WP_004150355.1 NZ_CP044258:c5359516-5359274 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT140499 NZ_CP059283:c2788700-2788332 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATTTGGACCAGGTCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References