Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2673284..2673935 Replicon chromosome
Accession NZ_CP044236
Organism Lacticaseibacillus paracasei strain WX322

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag F0M22_RS12915 Protein ID WP_003567661.1
Coordinates 2673284..2673667 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag F0M22_RS12920 Protein ID WP_003567665.1
Coordinates 2673687..2673935 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0M22_RS12910 2672579..2673100 - 522 WP_003567657.1 QueT transporter family protein -
F0M22_RS12915 2673284..2673667 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F0M22_RS12920 2673687..2673935 - 249 WP_003567665.1 antitoxin Antitoxin
F0M22_RS12925 2674003..2675139 - 1137 WP_003581082.1 alanine racemase -
F0M22_RS12930 2675126..2675500 - 375 WP_003567669.1 holo-ACP synthase -
F0M22_RS12935 2675669..2677177 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
F0M22_RS12940 2677303..2677446 - 144 WP_016380391.1 hypothetical protein -
F0M22_RS12945 2677477..2678865 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T140481 WP_003567661.1 NZ_CP044236:c2673667-2673284 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T140481 NZ_CP059281:c3677829-3677575 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT140481 WP_003567665.1 NZ_CP044236:c2673935-2673687 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT140481 NZ_CP059281:c3678077-3677826 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References