Detailed information of TA system TA140475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1922405..1923056
• Replicon: chromosome
• Accession: NZ_CP044228
• Organism: Lacticaseibacillus rhamnosus strain hsryfm 1301

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: F5976_RS10105
• Protein ID: WP_005686631.1
• Coordinates: 1922673..1923056 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: F5976_RS10100
• Protein ID: WP_005686632.1
• Coordinates: 1922405..1922653 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F5976_RS10080	1917454..1918842	+	1389	WP_005686636.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
F5976_RS10085	1919116..1920624	+	1509	WP_005686635.1	DEAD/DEAH box helicase	-
F5976_RS10090	1920793..1921167	+	375	WP_005686634.1	holo-ACP synthase	-
F5976_RS10095	1921154..1922293	+	1140	WP_005686633.1	alanine racemase	-
F5976_RS10100	1922405..1922653	+	249	WP_005686632.1	antitoxin	Antitoxin
F5976_RS10105	1922673..1923056	+	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F5976_RS10115	1923258..1923455	+	198	WP_064536013.1	hypothetical protein	-
F5976_RS10120	1923493..1924020	+	528	WP_005686630.1	QueT transporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T140475 WP_005686631.1 NZ_CP044228:1922673-1923056 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T140475 NZ_CP059281:2086445-2086747 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT140475 WP_005686632.1 NZ_CP044228:1922405-1922653 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT140475 NZ_CP059281:2086783-2087124 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19