Detailed information of TA system TA140472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT-HigA
• Location: 4070084..4070757
• Replicon: chromosome
• Accession: NZ_CP044222
• Organism: Nitrincola sp. KXZD1103

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F5I99_RS18860
• Protein ID: WP_151058735.1
• Coordinates: 4070084..4070347 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F5I99_RS18865
• Protein ID: WP_023012140.1
• Coordinates: 4070464..4070757 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F5I99_RS18840	4065294..4067348	-	2055	WP_191905900.1	marine proteobacterial sortase target protein	-
F5I99_RS18845	4067578..4068246	+	669	WP_151058729.1	two-component system response regulator CreB	-
F5I99_RS18850	4068243..4069688	+	1446	WP_151058731.1	two-component system sensor histidine kinase CreC	-
F5I99_RS18855	4069825..4070094	-	270	WP_151058733.1	CopG family transcriptional regulator	-
F5I99_RS18860	4070084..4070347	-	264	WP_151058735.1	BrnT family toxin	Toxin
F5I99_RS18865	4070464..4070757	-	294	WP_023012140.1	HigA family addiction module antidote protein	Antitoxin
F5I99_RS18870	4071038..4071259	+	222	WP_151058737.1	hypothetical protein	-
F5I99_RS18875	4071252..4071542	+	291	WP_151058739.1	type II toxin-antitoxin system RelE/ParE family toxin	-
F5I99_RS18880	4071689..4072732	+	1044	WP_151058741.1	IS110 family transposase	-
F5I99_RS19490	4072755..4072895	+	141	WP_191905901.1	hypothetical protein	-
F5I99_RS18885	4073286..4073771	-	486	WP_151058743.1	GNAT family N-acetyltransferase	-
F5I99_RS18890	4073902..4074270	-	369	WP_151058745.1	DUF3703 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4071689..4072732	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10386.76 Da
• Isoelectric Point: 6.8815

>T140472 WP_151058735.1 NZ_CP044222:c4070347-4070084 [Nitrincola sp. KXZD1103]
MNIFEFDDEKSQANRHKHGIDFHDAQVLWEDQDLLEVRAKSSDEPRYLVIGLIGMKHWSAVITYRGEKIRLISVRRSRKR
EVELYES

Nucleotide

• Length: 264 bp

>T140472 NZ_CP059281:1641401-1641715 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10770.33 Da
• Isoelectric Point: 6.2137

>AT140472 WP_023012140.1 NZ_CP044222:c4070757-4070464 [Nitrincola sp. KXZD1103]
MTMHNPPHPGEFIREVYLEPFGISSRQLASSLGVSPSTLSRLLKGDSGISPEMSLRLSKVLGRTPESWLAMQDMYDLWVA
RGTVNLDGIHRLDFEAA

Nucleotide

• Length: 294 bp

>AT140472 NZ_CP059281:1641165-1641398 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG