Detailed information of TA system TA140469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 2563903..2564413
• Replicon: chromosome
• Accession: NZ_CP044222
• Organism: Nitrincola sp. KXZD1103

Toxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: F5I99_RS11845
• Protein ID: WP_151056262.1
• Coordinates: 2563903..2564166 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: W9VFP3
• Locus tag: F5I99_RS11850
• Protein ID: WP_036513780.1
• Coordinates: 2564159..2564413 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F5I99_RS11815	2559421..2559789	-	369	WP_151056254.1	hypothetical protein	-
F5I99_RS11820	2560201..2560416	-	216	WP_151056256.1	TIGR02450 family Trp-rich protein	-
F5I99_RS11825	2560796..2561770	+	975	WP_151056258.1	DUF1852 domain-containing protein	-
F5I99_RS11830	2561798..2562829	+	1032	WP_151059141.1	methionine synthase	-
F5I99_RS11835	2562894..2563283	-	390	WP_151056260.1	4-oxalocrotonate tautomerase family protein	-
F5I99_RS11840	2563332..2563748	-	417	WP_151059143.1	MarR family transcriptional regulator	-
F5I99_RS11845	2563903..2564166	-	264	WP_151056262.1	Txe/YoeB family addiction module toxin	Toxin
F5I99_RS11850	2564159..2564413	-	255	WP_036513780.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
F5I99_RS11855	2564775..2565650	+	876	WP_151059145.1	DNA ligase	-
F5I99_RS11860	2565711..2566394	-	684	WP_151059147.1	AAA family ATPase	-
F5I99_RS11865	2566620..2567483	+	864	WP_151059149.1	alpha/beta fold hydrolase	-
F5I99_RS11870	2567424..2567864	-	441	WP_151056264.1	hypothetical protein	-
F5I99_RS11875	2567851..2569188	-	1338	WP_151056266.1	phosphatase PAP2/dual specificity phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10434.80 Da
• Isoelectric Point: 8.8660

>T140469 WP_151056262.1 NZ_CP044222:c2564166-2563903 [Nitrincola sp. KXZD1103]
MSSRLLSWTDESWTDYLYWQTQDKKTLKRINKLLNDVRRSPFEGIGKPEPLKENLAGFWSRRIDDTNRFVYAVDDTAITI
ISCRYHY

Nucleotide

• Length: 264 bp

>T140469 NZ_CP059281:1460771-1461148 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9382.56 Da
• Isoelectric Point: 5.0481

>AT140469 WP_036513780.1 NZ_CP044222:c2564413-2564159 [Nitrincola sp. KXZD1103]
MRIVSFTEARNSLKAVLDSVVNDADTAVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQFKAGKVTSRD
LIDE

Nucleotide

• Length: 255 bp

>AT140469 NZ_CP059281:1460314-1460682 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: W9VFP3