Detailed information of TA system TA140466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1602939..1603732
• Replicon: chromosome
• Accession: NZ_CP044222
• Organism: Nitrincola sp. KXZD1103

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: F5I99_RS07275
• Protein ID: WP_151054543.1
• Coordinates: 1602939..1603475 (-)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: F5I99_RS07280
• Protein ID: WP_151054545.1
• Coordinates: 1603466..1603732 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F5I99_RS07250	1598848..1599171	-	324	WP_151054534.1	helix-turn-helix domain-containing protein	-
F5I99_RS07255	1599378..1600178	-	801	WP_151054536.1	transglutaminase family protein	-
F5I99_RS07260	1600413..1600940	-	528	WP_151054537.1	GNAT family N-acetyltransferase	-
F5I99_RS07265	1600937..1601215	-	279	WP_151054539.1	DUF1778 domain-containing protein	-
F5I99_RS07270	1601356..1602903	-	1548	WP_151054541.1	plasmid pRiA4b ORF-3 family protein	-
F5I99_RS07275	1602939..1603475	-	537	WP_151054543.1	GNAT family N-acetyltransferase	Toxin
F5I99_RS07280	1603466..1603732	-	267	WP_151054545.1	DUF1778 domain-containing protein	Antitoxin
F5I99_RS07285	1603855..1604406	-	552	WP_151054547.1	pyridoxamine 5'-phosphate oxidase family protein	-
F5I99_RS07290	1604792..1605889	-	1098	WP_151054549.1	tyrosine-type recombinase/integrase	-
F5I99_RS07295	1605867..1606088	-	222	WP_151054551.1	helix-turn-helix domain-containing protein	-
F5I99_RS07300	1606078..1606344	-	267	WP_151054553.1	hypothetical protein	-
F5I99_RS07305	1606347..1606691	-	345	WP_151054555.1	hypothetical protein	-
F5I99_RS07310	1606688..1607059	-	372	WP_151054557.1	hypothetical protein	-
F5I99_RS07315	1607052..1607309	-	258	WP_151054559.1	hypothetical protein	-
F5I99_RS07320	1607309..1607689	-	381	WP_151054561.1	hypothetical protein	-
F5I99_RS07325	1607682..1608008	-	327	WP_151054563.1	hypothetical protein	-
F5I99_RS07330	1608001..1608621	-	621	WP_151054565.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-83)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19486.70 Da
• Isoelectric Point: 9.7197

>T140466 WP_151054543.1 NZ_CP044222:c1603475-1602939 [Nitrincola sp. KXZD1103]
MGISAPTLLTDQHQLNPFQCGIEELDIWLSKQALKSQKRGTARVYVVNDTKTRQVVGYYAIAMGSVSREHAFSSLRRNSP
EPIPMVILARLGVDSAYQGRGIAAGLLKDCILRSIQAMNAVGGAGILVHAIDPSAQSFYKKFGFKESALDPLVLMARICD
IEKTLTHHSLFLRFGLIG

Nucleotide

• Length: 537 bp

>T140466 NZ_CP059281:c478737-478630 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9954.46 Da
• Isoelectric Point: 5.1299

>AT140466 WP_151054545.1 NZ_CP044222:c1603732-1603466 [Nitrincola sp. KXZD1103]
MATKTAPINMRVLPSVRDIIDAAASLKKVDRTVFIQQAAINEAHNVLAEQRDFVLEAKAFEAFDLELRAEPQTLEGMKDL
FNRKAPWE

Nucleotide

• Length: 267 bp

>AT140466 NZ_CP059281:478786-478851 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT