Detailed information of TA system TA140462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1148485..1149056
• Replicon: chromosome
• Accession: NZ_CP044221
• Organism: Streptococcus mutans strain NCH105

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F5989_RS05705
• Protein ID: WP_002289429.1
• Coordinates: 1148485..1148817 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q8DW96
• Locus tag: F5989_RS05710
• Protein ID: WP_002262990.1
• Coordinates: 1148811..1149056 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F5989_RS05680	1143999..1144601	-	603	WP_002289786.1	NAD(P)H-dependent oxidoreductase	-
F5989_RS05685	1144743..1145591	-	849	WP_002265112.1	alpha/beta hydrolase	-
F5989_RS05690	1145569..1146789	-	1221	WP_002270688.1	PTS sugar transporter subunit IIC	-
F5989_RS05695	1146956..1147324	-	369	Protein_1073	transposase family protein	-
F5989_RS05700	1147489..1148280	+	792	WP_002289431.1	nucleotidyltransferase domain-containing protein	-
F5989_RS05705	1148485..1148817	-	333	WP_002289429.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F5989_RS05710	1148811..1149056	-	246	WP_002262990.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F5989_RS05715	1149378..1149770	-	393	WP_002262992.1	30S ribosomal protein S9	-
F5989_RS05720	1149795..1150241	-	447	WP_002262993.1	50S ribosomal protein L13	-
F5989_RS05725	1150640..1150834	-	195	WP_002262994.1	helix-turn-helix transcriptional regulator	-
F5989_RS05730	1150831..1151277	-	447	WP_002289426.1	hypothetical protein	-
F5989_RS05735	1151277..1151792	-	516	WP_002264864.1	hypothetical protein	-
F5989_RS05740	1151924..1152784	-	861	WP_002265709.1	DegV family protein	-
F5989_RS05745	1152808..1153554	-	747	WP_002264862.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1147196..1147324	128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12650.53 Da
• Isoelectric Point: 6.4634

>T140462 WP_002289429.1 NZ_CP044221:c1148817-1148485 [Streptococcus mutans]
MVTIKQGSVIKINLDPKQGHEQKGYRPYICLNHSIVTKYSNIAIFAPISNTKRDYPFYVPLEGTESTGKVLLDQLVTIDF
NARDYRYVEDIQEDLLDELLARVKVLFEKG

Nucleotide

• Length: 333 bp

>T140462 NZ_CP059281:c24220-23906 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9241.52 Da
• Isoelectric Point: 4.3021

>AT140462 WP_002262990.1 NZ_CP044221:c1149056-1148811 [Streptococcus mutans]
MQLVINKWGNSSAIRLPKQLVQELQLQTNDVLDYKVSGNKIILEKVNNIPELTVEDLFKDYQGEPVNVTPALFESVGNEQ
W

Nucleotide

• Length: 246 bp

>AT140462 NZ_CP059281:c23909-23493 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: Q8DW96