Detailed information of TA system TA140458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 622302..623793
• Replicon: chromosome
• Accession: NZ_CP044218
• Organism: Mesorhizobium terrae strain NIBRBAC000500504

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: FZF13_RS04215
• Protein ID: WP_065997438.1
• Coordinates: 622302..623180 (-)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: A0A1C2E0V7
• Locus tag: FZF13_RS04220
• Protein ID: WP_065997439.1
• Coordinates: 623173..623793 (-)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZF13_RS04195	618212..619057	+	846	WP_024924739.1	arylamine N-acetyltransferase	-
FZF13_RS04205	620276..621475	-	1200	WP_065997435.1	hypothetical protein	-
FZF13_RS04210	621472..622305	-	834	WP_065997437.1	hypothetical protein	-
FZF13_RS04215	622302..623180	-	879	WP_065997438.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FZF13_RS04220	623173..623793	-	621	WP_065997439.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-262)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (10-88)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 32288.06 Da
• Isoelectric Point: 4.7856

>T140458 WP_065997438.1 NZ_CP044218:c623180-622302 [Mesorhizobium terrae]
MAKAPTNMAKSVKDRLLNIARKEGRVFDILLVRFALERLLYRLSISAHQDRFVLKGGMLVTVWVTDDNRVTRDADFLGLG
DADPGRLIADFGEIMAAKSDDGLVFDVDALAATVIREEMEYGGIRLKTAAYLERTRIPVTIDIGVGDAMADATQRLDYPA
LLDFPAPQVRSYPPATVIAEKFQAMVALGVLNGRMKDYYDLWAIPRAIDISADDLDAAIRATFERRRTPIPTERPPGLSV
DMFGDEGKQRQWRAYAASLELDAVSLESIIEGVWELVGPSCARIVARNGNEA

Nucleotide

• Length: 879 bp

>T140458 NZ_CP059279:c4931901-4931794 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 22576.08 Da
• Isoelectric Point: 10.2501

>AT140458 WP_065997439.1 NZ_CP044218:c623793-623173 [Mesorhizobium terrae]
MPSAQDSQKAKLRSMLDARSMMHAHELREAGISAQTIARAVESGEVERISRGVYQKRGAEVEENQILAEAIMRVPKGVIA
LVSALAFHELTDQMPRRVWVAIGTSDWAPVQSYPPLRMVRFTAPYLRQGIEHHAIAGLDVPVYSIPKTLADLFRNPKLVD
RSVAVEGLRAALQQRKATPSAIAQAAKAGGAWKVMQPYIEALTSNG

Nucleotide

• Length: 621 bp

>AT140458 NZ_CP059279:4931950-4932015 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A1C2E0V7