Detailed information of TA system TA140455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 51570..52241
• Replicon: plasmid pVv180806
• Accession: NZ_CP044208
• Organism: Vibrio vulnificus strain Vv180806

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: F6X00_RS24975
• Protein ID: WP_011149008.1
• Coordinates: 51570..51869 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: Q7MBL1
• Locus tag: F6X00_RS24980
• Protein ID: WP_011149007.1
• Coordinates: 51945..52241 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6X00_RS24925	46614..46886	+	273	WP_182291738.1	hypothetical protein	-
F6X00_RS24930	46894..47373	+	480	WP_011149021.1	hypothetical protein	-
F6X00_RS24935	47393..47731	+	339	WP_011149019.1	hypothetical protein	-
F6X00_RS24940	47840..48136	+	297	WP_011149018.1	hypothetical protein	-
F6X00_RS24945	48717..49025	+	309	WP_094867925.1	hypothetical protein	-
F6X00_RS24950	49064..49321	+	258	WP_094867926.1	hypothetical protein	-
F6X00_RS24955	49874..50377	+	504	WP_011149015.1	hypothetical protein	-
F6X00_RS24960	50380..50688	+	309	WP_065091376.1	DUF1738 domain-containing protein	-
F6X00_RS24965	50756..51079	+	324	WP_094867927.1	type I restriction endonuclease subunit M	-
F6X00_RS24970	51314..51532	+	219	WP_065091378.1	hypothetical protein	-
F6X00_RS24975	51570..51869	+	300	WP_011149008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F6X00_RS24980	51945..52241	+	297	WP_011149007.1	putative addiction module antidote protein	Antitoxin
F6X00_RS24985	52832..54214	+	1383	WP_094889994.1	DUF3560 domain-containing protein	-
F6X00_RS24990	54296..54742	+	447	WP_011149004.1	lytic transglycosylase domain-containing protein	-
F6X00_RS24995	55309..55668	+	360	WP_103193725.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65566	65566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11134.96 Da
• Isoelectric Point: 10.6229

>T140455 WP_011149008.1 NZ_CP044208:51570-51869 [Vibrio vulnificus]
MIYNIKRTEIFNDWLRELKDPQARGSIAARVQRLTQGLNGDVKPVGAGISELRIHVGKGYRVYFKRSGKQIIVLLCGGNK
KTQNQDIELAKQLAQELGL

Nucleotide

• Length: 300 bp

>T140455 NZ_CP059279:4517447-4517911 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10437.90 Da
• Isoelectric Point: 4.6855

>AT140455 WP_011149007.1 NZ_CP044208:51945-52241 [Vibrio vulnificus]
MSKNTAMLADFDPAEYLDTPEAIQAYLDEAVASGDAAFISQSLGVIARAKGMTELAEQTGLSREALYRTLSQKGNPNFKG
FMAIISALGISLKLNVHA

Nucleotide

• Length: 297 bp

>AT140455 NZ_CP059279:4517112-4517447 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: Q7MBL1