Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 3586317..3586939 Replicon chromosome
Accession NZ_CP044205
Organism Candidatus Methylospira mobilis strain Shm1

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag F6R98_RS16330 Protein ID WP_153249962.1
Coordinates 3586646..3586939 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag F6R98_RS16325 Protein ID WP_153249961.1
Coordinates 3586317..3586631 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6R98_RS16310 3581913..3582419 + 507 WP_153249959.1 protein disulfide oxidoreductase -
F6R98_RS21695 3583119..3583307 + 189 WP_194269985.1 hypothetical protein -
F6R98_RS16315 3583375..3584897 + 1523 Protein_3232 IS21 family transposase -
F6R98_RS16320 3584887..3585693 + 807 WP_153249960.1 IS21-like element helper ATPase IstB -
F6R98_RS16325 3586317..3586631 - 315 WP_153249961.1 putative addiction module antidote protein Antitoxin
F6R98_RS16330 3586646..3586939 - 294 WP_153249962.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F6R98_RS21700 3587171..3587377 + 207 WP_194270264.1 hypothetical protein -
F6R98_RS16335 3587283..3587678 + 396 WP_153249963.1 tyrosine-type recombinase/integrase -
F6R98_RS16340 3587818..3588099 - 282 WP_153249964.1 HigA family addiction module antidote protein -
F6R98_RS16345 3588109..3588390 - 282 WP_153249965.1 type II toxin-antitoxin system RelE/ParE family toxin -
F6R98_RS16350 3588806..3589300 - 495 WP_153249966.1 cytochrome -
F6R98_RS16355 3589461..3590789 - 1329 WP_153251109.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-85)

Antitoxin

Cro/C1-type helix-turn-helix domain

(50-94)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10823.70 Da        Isoelectric Point: 10.6498

>T140441 WP_153249962.1 NZ_CP044205:c3586939-3586646 [Candidatus Methylospira mobilis]
MYIIKPLPNFTEWLDGLADEKTRGIISARIKRLERGLMGDVEPVGEGVSELRIHVGSGLRVYFTKRGARLIILLVGGSKS
TQKNDFKRAKQLATLLD

Download         Length: 294 bp

>T140441 NZ_CP059279:1095754-1096068 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11270.93 Da        Isoelectric Point: 5.1237

>AT140441 WP_153249961.1 NZ_CP044205:c3586631-3586317 [Candidatus Methylospira mobilis]
MTKRIKIDDLPEFDAAPYLSSEEAVAAYLTDILEANDAGLLAAALGDIARARGMTEISKLAGITREALYKALRPDSEPRI
DTINRVCSALGVRLVVQPTHLHPN

Download         Length: 315 bp

>AT140441 NZ_CP059279:1095518-1095751 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References