Detailed information of TA system TA140439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2350816..2351412
• Replicon: chromosome
• Accession: NZ_CP044205
• Organism: Candidatus Methylospira mobilis strain Shm1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: F6R98_RS10530
• Protein ID: WP_153251009.1
• Coordinates: 2351221..2351412 (-)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: F6R98_RS10525
• Protein ID: WP_153248980.1
• Coordinates: 2350816..2351211 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6R98_RS10520	2350394..2350807	+	414	WP_153248979.1	hypothetical protein	-
F6R98_RS10525	2350816..2351211	-	396	WP_153248980.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
F6R98_RS10530	2351221..2351412	-	192	WP_153251009.1	type II toxin-antitoxin system HicA family toxin	Toxin
F6R98_RS10535	2351679..2352527	+	849	WP_153248981.1	DUF4263 domain-containing protein	-
F6R98_RS10540	2352605..2353351	+	747	WP_153248982.1	hypothetical protein	-
F6R98_RS10545	2353581..2354072	+	492	WP_153248983.1	hypothetical protein	-
F6R98_RS10550	2354020..2355075	+	1056	WP_153248984.1	hypothetical protein	-
F6R98_RS10555	2355072..2355566	+	495	WP_153248985.1	adenylyltransferase/cytidyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2321320..2387876	66556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (7-62)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-125)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7217.15 Da
• Isoelectric Point: 10.8123

>T140439 WP_153251009.1 NZ_CP044205:c2351412-2351221 [Candidatus Methylospira mobilis]
ITTRDSKQVIKRLESEGWYLDRVRGSHHQFKHPTNPGRVTVKHPDSDIPAGALRSIEKQAGWK

Nucleotide

• Length: 192 bp

>T140439 NZ_CP059279:c946870-946613 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14399.15 Da
• Isoelectric Point: 4.5485

>AT140439 WP_153248980.1 NZ_CP044205:c2351211-2350816 [Candidatus Methylospira mobilis]
MRFPVVLHTDDGVRFGVTVPDLPGCFSAGDTFDDALNSVAEAIDLHLEGMTDDDEEIPVPRPIAEHRLNPDFEGGVWALV
DVDTARFDGRSEKINITLPRRLLAKIDDYARAHHSTRSGFLADAARLAMGK

Nucleotide

• Length: 396 bp

>AT140439 NZ_CP059279:c946601-946050 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA