Detailed information of TA system TA140438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 2103095..2103506
• Replicon: chromosome
• Accession: NZ_CP044205
• Organism: Candidatus Methylospira mobilis strain Shm1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: F6R98_RS09310
• Protein ID: WP_153248781.1
• Coordinates: 2103095..2103283 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: F6R98_RS09315
• Protein ID: WP_153248782.1
• Coordinates: 2103294..2103506 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6R98_RS09285	2098530..2099024	+	495	WP_153248778.1	hypothetical protein	-
F6R98_RS09290	2099488..2100831	+	1344	WP_153250985.1	group II intron reverse transcriptase/maturase	-
F6R98_RS09295	2100964..2101197	+	234	WP_153248779.1	addiction module protein	-
F6R98_RS09300	2101182..2101478	+	297	WP_153248780.1	type II toxin-antitoxin system RelE/ParE family toxin	-
F6R98_RS09305	2101851..2102891	-	1041	Protein_1846	IS110 family transposase	-
F6R98_RS09310	2103095..2103283	+	189	WP_153248781.1	type II toxin-antitoxin system HicA family toxin	Toxin
F6R98_RS09315	2103294..2103506	+	213	WP_153248782.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
F6R98_RS09320	2103935..2105017	-	1083	WP_153248775.1	IS91 family transposase	-
F6R98_RS09325	2105014..2105208	-	195	WP_153248776.1	tyrosine-type recombinase/integrase	-
F6R98_RS09330	2105181..2105921	-	741	WP_153248777.1	site-specific integrase	-
F6R98_RS09335	2106288..2106500	+	213	Protein_1852	transposase	-
F6R98_RS09340	2106551..2107435	-	885	WP_153248783.1	LysR family transcriptional regulator	-
F6R98_RS09345	2107551..2108492	+	942	WP_153248784.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2086786..2110070	23284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-62)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6801.96 Da
• Isoelectric Point: 10.9123

>T140438 WP_153248781.1 NZ_CP044205:2103095-2103283 [Candidatus Methylospira mobilis]
MKVKDLIALIESDGWWQIRQKGSHRQFHHPSKAGTVTVAGKLSIDVPPGTLLSVLKQAGLKQ

Nucleotide

• Length: 189 bp

>T140438 NZ_CP059279:915124-915501 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7561.53 Da
• Isoelectric Point: 4.0425

>AT140438 WP_153248782.1 NZ_CP044205:2103294-2103506 [Candidatus Methylospira mobilis]
MHYMVVIEEGPTSFGAYVPDLPGCIAVGESSQEALQLIQEAIELHIEELKSEGLNIPVPHSSSSFVEVHA

Nucleotide

• Length: 213 bp

>AT140438 NZ_CP059279:914667-915035 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA