Detailed information of TA system TA140423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 113372..113931
• Replicon: plasmid pAR-0423-2
• Accession: NZ_CP044160
• Organism: Shigella flexneri strain AR-0423

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2AJT2
• Locus tag: F4V35_RS24940
• Protein ID: WP_000421262.1
• Coordinates: 113656..113931 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A822PWA0
• Locus tag: F4V35_RS24935
• Protein ID: WP_011114774.1
• Coordinates: 113372..113656 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V35_RS24900	109297..109971	+	675	WP_004967157.1	IS66-like element accessory protein TnpA	-
F4V35_RS24905	109968..110315	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
F4V35_RS24910	110335..111936	+	1602	WP_011110603.1	IS66-like element ISSfl3 family transposase	-
F4V35_RS24915	112125..112310	+	186	Protein_139	ATP-binding protein	-
F4V35_RS24920	112410..112619	-	210	Protein_140	peptidoglycan-binding protein	-
F4V35_RS24925	112669..112920	-	252	WP_015683204.1	transporter	-
F4V35_RS24935	113372..113656	+	285	WP_011114774.1	ribbon-helix-helix domain-containing protein	Antitoxin
F4V35_RS24940	113656..113931	+	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V35_RS24955	114910..115863	+	954	Protein_145	IS66 family transposase	-
F4V35_RS24960	116399..116617	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	-
F4V35_RS24965	116619..116924	+	306	WP_001159860.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG	1..146462	146462
	-	inside	IScluster/Tn	-	ospI / ipaH9.8 / ospG	101623..123747	22124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10766.48 Da
• Isoelectric Point: 9.9324

>T140423 WP_000421262.1 NZ_CP044160:113656-113931 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T140423 NZ_CP059162:c2353268-2353086 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCACAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10529.97 Da
• Isoelectric Point: 6.2150

>AT140423 WP_011114774.1 NZ_CP044160:113372-113656 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR

Nucleotide

• Length: 285 bp

>AT140423 NZ_CP059162:c2353050-2352649 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAGCTGGCCCGTG
ACGGGCAAGCCATTCCCCTGCCCTCGCCCACCGGCACGCACCGCGCCAACCCCGACTTCGAGGGCATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTACGGGAGCACAACATCAAGAGCCGGTCGTCTTTCCTGGCGGATGCGGCGATGGAAAAGCTGGGACGTT
AA

Structures

Toxin

• AlphaFold DB: A0A822PVM3

Antitoxin

• AlphaFold DB: A0A822PWA0