Detailed information of TA system TA140422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 57650..58425
• Replicon: plasmid pAR-0423-2
• Accession: NZ_CP044160
• Organism: Shigella flexneri strain AR-0423

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: F4V35_RS24560
• Protein ID: WP_000405245.1
• Coordinates: 57650..58132 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: F4V35_RS24565
• Protein ID: WP_010921625.1
• Coordinates: 58123..58425 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V35_RS24545	54065..55756	+	1692	WP_000597733.1	T3SS effector E3 ubiquitin-protein ligase IpaH2.5	-
F4V35_RS24550	55808..57016	+	1209	Protein_75	IS3 family transposase	-
F4V35_RS24555	57059..57582	-	524	Protein_76	IS3-like element ISSfl11 family transposase	-
F4V35_RS24560	57650..58132	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
F4V35_RS24565	58123..58425	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
F4V35_RS24575	58697..58969	+	273	WP_000019158.1	hypothetical protein	-
F4V35_RS24580	58950..60158	+	1209	WP_000198552.1	IS91 family transposase	-
F4V35_RS24585	60330..60458	+	129	Protein_81	IS5/IS1182 family transposase	-
F4V35_RS24595	60607..62061	-	1455	WP_000701108.1	type 3 secretion system effector OspC2	-
F4V35_RS24600	62465..63084	+	620	Protein_83	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG	1..146462	146462
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ospC2	44954..82326	37372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T140422 WP_000405245.1 NZ_CP044160:c58132-57650 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T140422 NZ_CP059162:c2283511-2283233 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGATTATCAGTTTTCGCTGTGGCGACACCGAGACCCTGTTCACCACCGGATCCTCCCGAAAATGGAGGGCTATCCTGCC
TGTGGCACAACGGAAGCTGACCATGCTAGACCAGGCGATCGCATTACAGGACTTGCGTTCGCCGCCCGGCAATCGGCTGG
AAGCACTACAAGGGAACCGACAAGGCCAATACAGCATCCGAATCAATGATCAATATCGTATCTGCTTCACCTGGGATGCT
CAGGGGCCAGAAAACGTTGAAATTGTAGACTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT140422 WP_010921625.1 NZ_CP044160:c58425-58123 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT140422 NZ_CP059162:c2283224-2282919 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGTTTAATAACGGCATGCGCCCTGTACATCCGGGCGAAATTCTCAGGGAAGAATTTCTTCTCCCAATGGATTTAACGCC
GACGGCTCTTGCGAGAATGCTTCACGTATCCCCGCCAACGGTGAATGAAATTGTTCGTGAGAAACGCGGCATTACTGCTG
ACGTTGCGCTGCGTTTGGCTGCTTGCTTGAATACCAGCGCACAATTTTGGACGAATCTTCAGTCTGCGTATGATCTTCGT
CGAGCCGAAATTGACCATGGCGCGGATATTCTAAGGCAAGTTGAACGAGCCCCAGAGCCAGCTTAG

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8