Detailed information of TA system TA140417

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3384029..3384830
• Replicon: chromosome
• Accession: NZ_CP044158
• Organism: Shigella flexneri strain AR-0423

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: F4V35_RS17570
• Protein ID: WP_001094430.1
• Coordinates: 3384453..3384830 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: D3GUT4
• Locus tag: F4V35_RS17565
• Protein ID: WP_001285620.1
• Coordinates: 3384029..3384406 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V35_RS17525 (3380650)	3380650..3381105	+	456	WP_005051844.1	IrmA family protein	-
F4V35_RS25875 (3381248)	3381248..3381337	+	90	WP_112031555.1	DUF905 family protein	-
F4V35_RS17540 (3381516)	3381516..3382334	+	819	WP_001177758.1	DUF932 domain-containing protein	-
F4V35_RS17550 (3382676)	3382676..3383149	+	474	WP_000855059.1	antirestriction protein	-
F4V35_RS17555 (3383165)	3383165..3383641	+	477	WP_001387238.1	RadC family protein	-
F4V35_RS17560 (3383728)	3383728..3383949	+	222	WP_000692350.1	DUF987 domain-containing protein	-
F4V35_RS17565 (3384029)	3384029..3384406	+	378	WP_001285620.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
F4V35_RS17570 (3384453)	3384453..3384830	+	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
F4V35_RS17575 (3384827)	3384827..3385315	+	489	WP_000761715.1	DUF5983 family protein	-
F4V35_RS17580 (3385335)	3385335..3385532	+	198	WP_000772029.1	DUF957 domain-containing protein	-
F4V35_RS17585 (3385617)	3385617..3386459	+	843	WP_001290187.1	DUF4942 domain-containing protein	-
F4V35_RS17590 (3387235)	3387235..3387480	+	246	WP_000875412.1	hypothetical protein	-
F4V35_RS17595 (3387883)	3387883..3389045	+	1163	WP_094081514.1	IS3-like element IS3 family transposase	-
F4V35_RS17600 (3389149)	3389149..3389616	-	468	WP_011069513.1	prepilin peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T140417 WP_001094430.1 NZ_CP044158:3384453-3384830 [Shigella flexneri]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T140417 NZ_CP059156:c2199786-2199577 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13698.52 Da
• Isoelectric Point: 5.9505

>AT140417 WP_001285620.1 NZ_CP044158:3384029-3384406 [Shigella flexneri]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT140417 NZ_CP059156:c2200091-2199798 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7

Antitoxin

• AlphaFold DB: A0A0E0Y522