Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3261669..3262323 Replicon chromosome
Accession NZ_CP044158
Organism Shigella flexneri strain AR-0423

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag F4V35_RS16935 Protein ID WP_000244767.1
Coordinates 3261669..3262076 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag F4V35_RS16940 Protein ID WP_000354052.1
Coordinates 3262057..3262323 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V35_RS16915 (3257627) 3257627..3259359 - 1733 Protein_3248 single-stranded-DNA-specific exonuclease RecJ -
F4V35_RS16920 (3259365) 3259365..3260075 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F4V35_RS16925 (3260100) 3260100..3260996 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
F4V35_RS16930 (3261108) 3261108..3261629 + 522 WP_001055867.1 flavodoxin FldB -
F4V35_RS16935 (3261669) 3261669..3262076 - 408 WP_000244767.1 protein YgfX Toxin
F4V35_RS16940 (3262057) 3262057..3262323 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
F4V35_RS16945 (3262566) 3262566..3263546 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
F4V35_RS16950 (3263742) 3263742..3264401 - 660 WP_000250274.1 hemolysin III family protein -
F4V35_RS16955 (3264565) 3264565..3264875 - 311 Protein_3256 N(4)-acetylcytidine aminohydrolase -
F4V35_RS16960 (3264920) 3264920..3266353 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
F4V35_RS16965 (3266410) 3266410..3267153 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T140416 WP_000244767.1 NZ_CP044158:c3262076-3261669 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T140416 NZ_CP059156:c2179204-2178842 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT140416 WP_000354052.1 NZ_CP044158:c3262323-3262057 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT140416 NZ_CP059156:c2179371-2179201 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References