Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/ElaA-DUF1778 |
| Location | 200754..201511 | Replicon | plasmid pAR-0424-2 |
| Accession | NZ_CP044157 | ||
| Organism | Shigella flexneri strain AR-0424 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | D2AJG5 |
| Locus tag | F4V36_RS25330 | Protein ID | WP_000501974.1 |
| Coordinates | 201026..201511 (+) | Length | 162 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | Q31SN8 |
| Locus tag | F4V36_RS25325 | Protein ID | WP_011114751.1 |
| Coordinates | 200754..201038 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| F4V36_RS25290 | 196815..197489 | + | 675 | WP_004967157.1 | IS66-like element accessory protein TnpA | - |
| F4V36_RS25295 | 197486..197836 | + | 351 | WP_005048975.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| F4V36_RS25300 | 197833..199434 | + | 1602 | WP_005063916.1 | IS66-like element ISSfl3 family transposase | - |
| F4V36_RS25810 | 199688..199852 | - | 165 | WP_001346193.1 | hypothetical protein | - |
| F4V36_RS25325 | 200754..201038 | + | 285 | WP_011114751.1 | DUF1778 domain-containing protein | Antitoxin |
| F4V36_RS25330 | 201026..201511 | + | 486 | WP_000501974.1 | GNAT family N-acetyltransferase | Toxin |
| F4V36_RS25335 | 201684..201965 | - | 282 | Protein_239 | IS4/IS5 family transposase | - |
| F4V36_RS25340 | 202032..203188 | + | 1157 | WP_094085527.1 | IS3-like element IS600 family transposase | - |
| F4V36_RS25345 | 203258..204531 | + | 1274 | WP_094081497.1 | IS3-like element IS2 family transposase | - |
| F4V36_RS25350 | 204569..204712 | + | 144 | Protein_242 | DUF3440 domain-containing protein | - |
| F4V36_RS25355 | 204697..205335 | + | 639 | WP_039065741.1 | ParB N-terminal domain-containing protein | - |
| F4V36_RS25360 | 205563..205987 | + | 425 | Protein_244 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ipaH7.8 / ospC2 / ipaH2.5 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ospD3/senA / ospD3/senA / ipaH4.5 | 1..226601 | 226601 | |
| - | inside | IScluster/Tn | - | ospC3 / ospC1 / ospD3/senA / ospD3/senA / ospD3/senA | 188826..225025 | 36199 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 162 a.a. Molecular weight: 17814.63 Da Isoelectric Point: 9.8822
>T140396 WP_000501974.1 NZ_CP044157:201026-201511 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q
Download Length: 486 bp
>T140396 NZ_CP059155:c871840-871652 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10871.52 Da Isoelectric Point: 4.6812
>AT140396 WP_011114751.1 NZ_CP044157:200754-201038 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV
Download Length: 285 bp
>AT140396 NZ_CP059155:c872129-871869 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PN28 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4V1CUJ1 |