Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 18978..19753 Replicon plasmid pAR-0424-2
Accession NZ_CP044157
Organism Shigella flexneri strain AR-0424

Toxin (Protein)


Gene name tacT Uniprot ID Q7BEG9
Locus tag F4V36_RS24110 Protein ID WP_000405245.1
Coordinates 19271..19753 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag F4V36_RS24105 Protein ID WP_010921625.1
Coordinates 18978..19280 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V36_RS24070 14320..14939 - 620 Protein_12 IS1 family transposase -
F4V36_RS24075 15343..16797 + 1455 WP_000701108.1 type 3 secretion system effector OspC2 -
F4V36_RS24085 16946..17074 - 129 Protein_14 IS5/IS1182 family transposase -
F4V36_RS24095 18434..18706 - 273 WP_000019158.1 hypothetical protein -
F4V36_RS24105 18978..19280 + 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
F4V36_RS24110 19271..19753 + 483 WP_000405245.1 GNAT family N-acetyltransferase Toxin
F4V36_RS24115 19821..20344 + 524 Protein_19 IS3-like element ISSfl11 family transposase -
F4V36_RS24120 20387..21596 - 1210 Protein_20 IS3 family transposase -
F4V36_RS24125 21648..23339 - 1692 WP_151106460.1 IpaH family type III secretion system E3 ubiquitin--protein ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ospC2 / ipaH2.5 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ospD3/senA / ospD3/senA / ipaH4.5 1..226601 226601
- inside IScluster/Tn - ospC2 / ipaH2.5 / ospE2 11961..32450 20489


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17750.52 Da        Isoelectric Point: 7.0382

>T140392 WP_000405245.1 NZ_CP044157:19271-19753 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T140392 NZ_CP059131:c94344-94210 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT140392 WP_010921625.1 NZ_CP044157:18978-19280 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT140392 NZ_CP059131:94389-94449 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q7BEG9

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References