Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1349971..1350772 Replicon chromosome
Accession NZ_CP044155
Organism Shigella flexneri strain AR-0424

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag F4V36_RS06955 Protein ID WP_001094430.1
Coordinates 1350395..1350772 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag F4V36_RS06950 Protein ID WP_001285620.1
Coordinates 1349971..1350348 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V36_RS06910 (1346592) 1346592..1347047 + 456 WP_005051844.1 IrmA family protein -
F4V36_RS25985 (1347190) 1347190..1347279 + 90 WP_112031555.1 DUF905 family protein -
F4V36_RS06925 (1347458) 1347458..1348276 + 819 WP_001177758.1 DUF932 domain-containing protein -
F4V36_RS06935 (1348618) 1348618..1349091 + 474 WP_000855059.1 antirestriction protein -
F4V36_RS06940 (1349107) 1349107..1349583 + 477 WP_001387238.1 RadC family protein -
F4V36_RS06945 (1349670) 1349670..1349891 + 222 WP_000692350.1 DUF987 domain-containing protein -
F4V36_RS06950 (1349971) 1349971..1350348 + 378 WP_001285620.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
F4V36_RS06955 (1350395) 1350395..1350772 + 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
F4V36_RS06960 (1350769) 1350769..1351257 + 489 WP_000761715.1 DUF5983 family protein -
F4V36_RS06965 (1351277) 1351277..1351474 + 198 WP_000772029.1 DUF957 domain-containing protein -
F4V36_RS06970 (1351559) 1351559..1352401 + 843 WP_001290187.1 DUF4942 domain-containing protein -
F4V36_RS06975 (1353178) 1353178..1353423 + 246 WP_000875412.1 hypothetical protein -
F4V36_RS06980 (1353826) 1353826..1354988 + 1163 WP_094081514.1 IS3-like element IS3 family transposase -
F4V36_RS06985 (1355092) 1355092..1355559 - 468 WP_011069513.1 prepilin peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T140376 WP_001094430.1 NZ_CP044155:1350395-1350772 [Shigella flexneri]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T140376 NZ_CP059130:c1264135-1263917 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT140376 WP_001285620.1 NZ_CP044155:1349971-1350348 [Shigella flexneri]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT140376 NZ_CP059130:c1264535-1264161 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References