Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3494074..3494728 Replicon chromosome
Accession NZ_CP044152
Organism Shigella flexneri strain AR-0425

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag F4V37_RS17795 Protein ID WP_000244767.1
Coordinates 3494321..3494728 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID Q0T100
Locus tag F4V37_RS17790 Protein ID WP_000354044.1
Coordinates 3494074..3494340 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V37_RS17765 (3489244) 3489244..3489987 + 744 WP_000951957.1 SDR family oxidoreductase -
F4V37_RS17770 (3490044) 3490044..3491477 - 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
F4V37_RS17775 (3491522) 3491522..3491832 + 311 Protein_3424 N(4)-acetylcytidine aminohydrolase -
F4V37_RS17780 (3491996) 3491996..3492655 + 660 WP_000250274.1 hemolysin III family protein -
F4V37_RS17785 (3492851) 3492851..3493831 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
F4V37_RS17790 (3494074) 3494074..3494340 + 267 WP_000354044.1 FAD assembly factor SdhE Antitoxin
F4V37_RS17795 (3494321) 3494321..3494728 + 408 WP_000244767.1 protein YgfX Toxin
F4V37_RS17800 (3494768) 3494768..3495289 - 522 WP_001055867.1 flavodoxin FldB -
F4V37_RS17805 (3495401) 3495401..3496297 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
F4V37_RS17810 (3496322) 3496322..3497032 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F4V37_RS17815 (3497038) 3497038..3498771 + 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T140357 WP_000244767.1 NZ_CP044152:3494321-3494728 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T140357 NZ_CP059125:c3582618-3582307 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10529.09 Da        Isoelectric Point: 5.1599

>AT140357 WP_000354044.1 NZ_CP044152:3494074-3494340 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT140357 NZ_CP059125:c3582310-3581891 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TIU2

References