Detailed information of TA system TA140336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4169722..4170559
• Replicon: chromosome
• Accession: NZ_CP044151
• Organism: Shigella sonnei strain AR-0426

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: F4V38_RS21460
• Protein ID: WP_000227784.1
• Coordinates: 4169722..4170264 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: F4V38_RS21465
• Protein ID: WP_001297137.1
• Coordinates: 4170248..4170559 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V38_RS21430 (4164786)	4164786..4165277	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
F4V38_RS21435 (4165405)	4165405..4166769	-	1365	WP_001000971.1	MFS transporter	-
F4V38_RS21440 (4166888)	4166888..4167585	+	698	WP_237158176.1	IS1-like element IS1A family transposase	-
F4V38_RS21445 (4167954)	4167954..4168352	+	399	Protein_4145	SEL1-like repeat protein	-
F4V38_RS21455 (4169100)	4169100..4169666	+	567	Protein_4147	SEL1-like repeat protein	-
F4V38_RS21460 (4169722)	4169722..4170264	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
F4V38_RS21465 (4170248)	4170248..4170559	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
F4V38_RS21470 (4170744)	4170744..4171634	-	891	WP_000971327.1	heme o synthase	-
F4V38_RS21475 (4171646)	4171646..4171975	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
F4V38_RS21480 (4171975)	4171975..4172589	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
F4V38_RS21485 (4172579)	4172579..4174570	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
F4V38_RS21490 (4174592)	4174592..4175539	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T140336 WP_000227784.1 NZ_CP044151:c4170264-4169722 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T140336 NZ_CP059119:3605774-3605992 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT140336 WP_001297137.1 NZ_CP044151:c4170559-4170248 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT140336 NZ_CP059119:3605374-3605748 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3