Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3431589..3432086 Replicon chromosome
Accession NZ_CP044151
Organism Shigella sonnei strain AR-0426

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F4V38_RS17770 Protein ID WP_001173343.1
Coordinates 3431913..3432086 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F4V38_RS17765 Protein ID WP_000398619.1
Coordinates 3431589..3431885 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V38_RS17745 (3428320) 3428320..3429039 - 720 WP_000611285.1 two-component system response regulator DcuR -
F4V38_RS17750 (3429036) 3429036..3430667 - 1632 WP_001216495.1 sensor histidine kinase -
F4V38_RS17755 (3430848) 3430848..3431078 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
F4V38_RS17760 (3431090) 3431090..3431362 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
F4V38_RS17765 (3431589) 3431589..3431885 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F4V38_RS17770 (3431913) 3431913..3432086 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F4V38_RS17775 (3432205) 3432205..3433722 - 1518 WP_001295086.1 lysine--tRNA ligase -
F4V38_RS17780 (3433959) 3433959..3435416 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
F4V38_RS17785 (3435475) 3435475..3435732 - 258 Protein_3423 lysine decarboxylase LdcC -
F4V38_RS17795 (3435778) 3435778..3436488 - 711 Protein_3424 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140334 WP_001173343.1 NZ_CP044151:3431913-3432086 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140334 NZ_CP059119:2743942-2744049 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT140334 WP_000398619.1 NZ_CP044151:3431589-3431885 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140334 NZ_CP059119:c2743895-2743829 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References