140318

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 41432..41957 Replicon plasmid pAR-0427-2
Accession NZ_CP044150
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag F4V39_RS29015 Protein ID WP_001159868.1
Coordinates 41432..41737 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag F4V39_RS29020 Protein ID WP_000813634.1
Coordinates 41739..41957 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS28985 36520..37686 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
F4V39_RS28995 38273..38635 - 363 Protein_40 RepB family plasmid replication initiator protein -
F4V39_RS29000 38689..39902 + 1214 WP_162829202.1 IS3 family transposase -
F4V39_RS29010 40625..41431 - 807 WP_000016989.1 site-specific integrase -
F4V39_RS29015 41432..41737 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
F4V39_RS29020 41739..41957 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F4V39_RS29025 42416..43099 + 684 WP_010891291.1 DNA-binding protein -
F4V39_RS29030 43096..43716 + 621 WP_001248529.1 hypothetical protein -
F4V39_RS29035 43713..43913 + 201 WP_000708307.1 hypothetical protein -
F4V39_RS29045 44320..45297 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
F4V39_RS29050 45582..46322 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
F4V39_RS29055 46443..46673 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / toxB / toxB / toxB / toxB / hlyD / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP 1..85887 85887


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T140318 WP_001159868.1 NZ_CP044150:c41737-41432 [Escherichia coli O157]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T140318 NZ_CP059091:69534-69878 [Pantoea agglomerans]
ATGGACGCACTATTCATCGAACTTTCCAGCTTCCAGAAATACCGGGCTGATTATCTGAATGATGATCAGTACAGGCTGTT
TCAGAACATGTTGCTTGCTGACCCTGAGAAGGGCGATGTGATACCCGATACCGGCGGACTGCGTAAAGTTCGCTTCAGGG
ATGAGCGCCGGCACAAGGGCGCACGTGGCGGGATCAGAGTTATCTATTACTGGACCAACACACAAGGCCAATTCATCCTG
TTTACCATCTACGATAAAGATCAACGCGACGATTTGACCAGGCAACAGCGTGATGCCCTGGCTACCGCGCTGAATGTGAT
CAAAAAAGGGCTGAGACATGACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT140318 WP_000813634.1 NZ_CP044150:c41957-41739 [Escherichia coli O157]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT140318 NZ_CP059091:69871-70185 [Pantoea agglomerans]
ATGACTGAACGCGATATCTTCAGTGAACTGATGACAGGTATGCAGGAGCTTAAGGATCATCAGGAGGGCAAAATTACGCT
TAAGAGTTATAAGGTCACTAAGCGTGCCCCTGTAACTATTGCGCCGCAGGAGTTACGCGACGTACGCGAAAAACTGAATC
TCTCACAGGCCGTATTCGCCCGTTATCTTCATACTGGTGAAACAACATATCAAAACTGGGAACAGGGCAGGGCCAGACCT
AACGCCCAGGCTGTTTTACTGATCCGCATGGTGCAGCAGAACCCAGAAACGCTGAAGACGCTCGCGAAGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References