Detailed information of TA system TA140313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 20912..21342
• Replicon: plasmid pAR-0427-1
• Accession: NZ_CP044149
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: F4V39_RS28385
• Protein ID: WP_001388374.1
• Coordinates: 20912..21076 (-)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: F4V39_RS28390
• Protein ID: WP_000079939.1
• Coordinates: 21073..21342 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS29630	16600..17046	-	447	Protein_24	DUF3363 domain-containing protein	-
F4V39_RS28365	17515..19008	-	1494	WP_001120888.1	IS91-like element ISVsa3 family transposase	-
F4V39_RS28370	19120..19500	-	381	WP_001696636.1	HTH domain-containing protein	-
F4V39_RS28380	19941..20864	-	924	WP_001415483.1	hypothetical protein	-
F4V39_RS28385	20912..21076	-	165	WP_001388374.1	hypothetical protein	Toxin
F4V39_RS28390	21073..21342	-	270	WP_000079939.1	hypothetical protein	Antitoxin
F4V39_RS29445	21415..21555	-	141	WP_021503378.1	hypothetical protein	-
F4V39_RS28400	22257..23111	-	855	Protein_31	incFII family plasmid replication initiator RepA	-
F4V39_RS28405	23104..23178	-	75	WP_001375168.1	RepA leader peptide Tap	-
F4V39_RS28415	23423..23670	-	248	Protein_33	replication regulatory protein RepA	-
F4V39_RS28420	23910..24500	-	591	WP_000766807.1	DUF2726 domain-containing protein	-
F4V39_RS28425	24538..24747	-	210	WP_001333231.1	hemolysin expression modulator Hha	-
F4V39_RS28430	24793..25254	-	462	WP_000978819.1	thermonuclease family protein	-
F4V39_RS28435	25499..25711	-	213	WP_001301409.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA8 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / tet(B)	-	1..74819	74819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T140313 WP_001388374.1 NZ_CP044149:c21076-20912 [Escherichia coli O157]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T140313 NZ_CP059089:2153983-2155296 [Pantoea agglomerans]
ATGCAAAGGCTGATTGCGTGGATGAACGGCGAGCGGGTAGGCGTACTCACCCGCCAGCGAAACGGCGCTCATGAGTTTCA
GTATGATACCGCCTGGCTGAGTAATCCGCTCTCCAGGCCGCTGTCACTTTCGATGCCGTTGCAGGTTAAAGCGATCACCT
CACACGCTGTGATTAACTTCTTCGATAACCTGCTGCCGGATAGCCCATTGATTCGCGACAGAATCGTGGCGCGTTACCAG
ATTAAATCCCGTCAGCCGTTTGATCTGTTGCAGGAAGTGGGACGTGACAGCGTCGGGGCAACCATGCTGCTGCCGCCCGA
TATGCCACTGCCCGCACTGCAGGCCCGCTATGAAGTGCTGGATGAAACCCGGCTGGAAAGCGTGCTGTCGGCCTATCAGT
CAGATATCCCGCTGGGGATGCTGCGTGAAGAGACCGATTTCCGTATCTCGGTCGCGGGGGCGCAGGAGAAAACTGCACTG
CTGCTGACCGATGAAGGCTGGAGCATTCCTGCCGGTGCCACGCCCACCTCGCACATCATTAAGCTGCCGATCGGAGAGAT
TAAACAGCCGGCCGCGACGCTGGATATGCGCGAAAGTGTGGAGAATGAGTATCTCTGTCTGGCGCTGGCCCGTGCACTCG
GTATCGACGTGCCGCAGGCAACGATTATCCGTGCCGGACAGATGCGCGCCCTGGCGGTAGAACGTTTCGACCGCCGCTGG
TCACGCGATAAAACCCGGTTGATCCGCCTGCCGCAGGAGGATTTCTGTCAGGCGCTGGGACTGCCATCTTCAACCAAATA
TGAGTCGGATGGCGGGCCGGGCATTGCGGAGATCATGCAGATACTGATGGGATCGAGCAACGCCATCGAAGATCGCTATC
GCTTTATGATGTTCCAGGTGTTTCAGTGGCTGATTGGCGCAACCGACGGTCACGCCAAGAACTTCTCACTGTTTATTGAG
CGCGGCGGCAGTTATCGACTGACTCCCTTTTATGATGTGATGTCCGGTTACCCGGTACTGGGCGGCACCGGTCTGAACAA
GCGAGCGCTGAAACTGGCGATGGGTCTGCGTGCCACTAAGGGGAAGAAGACAGAGATCGACAAAATCTTCCCGCGCCATT
TTATCGCCACGGCAAAAAGCGTCAGTTTTGATCGTGACAGTTTGCAGCAGATCTTCGACTTCTTTATTAAGGCCTTTCCC
CAGGCGGTGGTCTCGGTGCGCGATGCTTTGCCGCCAGATTTCCCGCAGCACATCGCCGACGCCATTTTCAGTCATTCACT
GATTATGCTGGAGCGGCTGCGGCACAAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT140313 WP_000079939.1 NZ_CP044149:c21342-21073 [Escherichia coli O157]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT140313 NZ_CP059089:2153735-2153983 [Pantoea agglomerans]
ATGATATACAGCCCGCTACAGCTGGCAAACCGGCTAAAGCTCATTCGTCAGCGCAATGGCTGGACCCAGAGTGAGCTGGC
GAAAAAGGTCGGCCTGAAACAGGCTACCATCTCTCACTTTGAAAATGCCCCTGACAGCACCTCGCTGGCAACGCTGTTTA
AGCTGTTACAGTCGCTGGAGCTGAGTCTGGAAGTTCAGGAGAAAGAGGAGGCCAGTGCGCCTCAGCTGCATGATGAGGAT
GAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2