Detailed information of TA system TA140307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 4920861..4921339
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: F4V39_RS24590
• Protein ID: WP_001303876.1
• Coordinates: 4920861..4921148 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: F4V39_RS24595
• Protein ID: WP_000536233.1
• Coordinates: 4921148..4921339 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS24550	4916010..4918481	-	2472	WP_000034474.1	exonuclease	-
F4V39_RS24555	4918575..4918766	-	192	WP_001098307.1	DUF1482 family protein	-
F4V39_RS24560	4918763..4918951	-	189	WP_000413705.1	cell division inhibition protein DicB	-
F4V39_RS24570	4919525..4919710	+	186	WP_001133046.1	hypothetical protein	-
F4V39_RS24575	4919897..4920286	-	390	WP_000394511.1	hypothetical protein	-
F4V39_RS24580	4920428..4920583	-	156	WP_000379575.1	DUF1391 family protein	-
F4V39_RS24590	4920861..4921148	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V39_RS24595	4921148..4921339	-	192	WP_000536233.1	hypothetical protein	Antitoxin
F4V39_RS24600	4921367..4921768	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
F4V39_RS24605	4921877..4922149	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
F4V39_RS24610	4922133..4922558	+	426	WP_000693855.1	Rha family transcriptional regulator	-
F4V39_RS24615	4922765..4923220	-	456	WP_000273724.1	hypothetical protein	-
F4V39_RS24620	4923299..4924390	+	1092	WP_001205823.1	hypothetical protein	-
F4V39_RS24625	4924397..4925143	+	747	WP_000788745.1	ATP-binding protein	-
F4V39_RS24630	4925165..4925935	+	771	WP_000450992.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T140307 WP_001303876.1 NZ_CP044148:c4921148-4920861 [Escherichia coli O157]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T140307 NZ_CP059088:c22950-22777 [Pantoea agglomerans]
TTGAATAGTGCACAACTCATGAAACTATTGAAGAGAAACGGCTGGGTTTTGGATCGTGTGAATGGGAGCCATCACATTTT
TGTGAAAGAAGGTGTTGACCATCATATTACGGTTCCCCACCCGAGGAAGGACTTAGCAAAAGGCACGCTACAGGACATCC
TGAAAAATATGTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT140307 WP_000536233.1 NZ_CP044148:c4921339-4921148 [Escherichia coli O157]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT140307 NZ_CP059088:c22706-22299 [Pantoea agglomerans]
ATGAAATTTCCGATCTATCTCCACAAGGCAGAAGGCGGCAGTTTCTCTGGCTTCGTACCAGACATTAAGGGCTGTTATTT
TGCCGGTGATAATATCGACGACACTATCGCTGATGCGTTTAGCGCCATTGACGCGCACCTGGAGTTCCAGGCAGAGAAAG
GAAAAGCTTTACCAACAGCTATGCCAGTAGAAGCACATTTCGATGATGAAAACTGCCAGGGGGGTTATTGGGCCTTCGTT
GATATTGATTTATCAAAGTACGATGGAAAAGCGGTGAAGCTGAACATTACGTTACCGCAAAATCTATTGAAAAAGATTGA
TAGCTACGTCGAGTTACATAAGGATTTTGGATCACGCAGTGGGTTTATAGCAGAGATAGCGCGGCGCGAACTGCAGAAAA
CTGCATAA