Detailed information of TA system TA140306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4745377..4746082
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: F4V39_RS23750
• Protein ID: WP_000539519.1
• Coordinates: 4745696..4746082 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: F4V39_RS23745
• Protein ID: WP_001280945.1
• Coordinates: 4745377..4745706 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS23730	4740534..4741445	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
F4V39_RS23735	4741623..4743971	+	2349	WP_000950320.1	EAL domain-containing protein	-
F4V39_RS23740	4743979..4745307	+	1329	WP_000086905.1	GGDEF domain-containing protein	-
F4V39_RS23745	4745377..4745706	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
F4V39_RS23750	4745696..4746082	-	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V39_RS23755	4746308..4747633	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
F4V39_RS23760	4747846..4748229	+	384	WP_000497137.1	biofilm formation regulator BssR	-
F4V39_RS23765	4748340..4749455	+	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
F4V39_RS23770	4749452..4750078	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T140306 WP_000539519.1 NZ_CP044148:c4746082-4745696 [Escherichia coli O157]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T140306 NZ_CP059087:79248-79562 [Pantoea agglomerans]
ATGCAGTTCACTGTATACAGTAATACTGGGAAAAGCGCCGTCTATCCCCTGCTGCTCGATGTTACCAGCGATATTATCGG
GCAGTTGAACCGCAGGATTGTGATCCCCTTGCTGCCTCTTGAAAAGTACCCGAGCAGCACGCGACCTGAACGGCTGGTTC
CGCTTATCAGACTGACTGATGACAAAGAATATGCCGTAATGACGCATGAAATGGCGAGCATTCCCACTCGTGCTTTGGGG
GCTGTGTTCTGCGATTCATCTCTCTATCGTACAGAGATTAAAGCTGCTATTGATTTTCTTCTCGACGGTATCTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT140306 WP_001280945.1 NZ_CP044148:c4745706-4745377 [Escherichia coli O157]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT140306 NZ_CP059087:79009-79245 [Pantoea agglomerans]
ATGGCTATAACACAACGAGCAACGCAAAGCGTTACCATGACCGTAGAACGTGACTTACTCAGCCGTGCGCGTGAGGCCGG
AATTAATCTCAGTGCTACCCTGACCACGGCCCTCGACGCTGAACTTCGTCAGTACGAGGCAAAAAAATGGCAGGAGGAAA
ACAGCGAAGCACTGGAAGCTCTAAACCGCTTTCACGATGAACACGGTTGTTTCAGCGATGAGTACAGGACATTTTAA