Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4290647..4291265 Replicon chromosome
Accession NZ_CP044148
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F4V39_RS21605 Protein ID WP_001291435.1
Coordinates 4290647..4290865 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag F4V39_RS21610 Protein ID WP_000344800.1
Coordinates 4290891..4291265 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS21570 4285934..4286506 + 573 WP_000779831.1 YbaY family lipoprotein -
F4V39_RS21575 4286539..4286850 - 312 WP_000409911.1 MGMT family protein -
F4V39_RS21585 4287229..4287582 + 354 WP_000878140.1 DUF1428 domain-containing protein -
F4V39_RS21590 4287624..4289174 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F4V39_RS21595 4289338..4289808 - 471 WP_000136192.1 YlaC family protein -
F4V39_RS21600 4289924..4290475 - 552 WP_000102553.1 maltose O-acetyltransferase -
F4V39_RS21605 4290647..4290865 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
F4V39_RS21610 4290891..4291265 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
F4V39_RS21615 4291811..4294960 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
F4V39_RS21620 4294983..4296176 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140305 WP_001291435.1 NZ_CP044148:c4290865-4290647 [Escherichia coli O157]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140305 NZ_CP059084:3387676-3387891 [Pantoea ananatis]
ATGAACAAAACCCTAACTAAAACTGATTATTTGATGCGACTGAGGCGCTGTCGTTCTATTGACACGCTGGAACGCGTGAT
TGAAAAAAATAAGTACGAATTACCCGATGAAGAATTAGCAGTATTTTACTCAGCAGCCGATCACCGCCTGGCTGAACTGA
CGATGAACAAGCTCTATGACAAAGTCCCGGTGTCAGTCTGGAAATTTGTACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT140305 WP_000344800.1 NZ_CP044148:c4291265-4290891 [Escherichia coli O157]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140305 NZ_CP059084:3387274-3387651 [Pantoea ananatis]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACTTGTGTGAGAATCTGTTTGACGACAGCATGGC
GACATTAACTGACAGTCATCACGGCTGGGTTAATGACCCCACTGCCGAAAGCAATTTACAGCTAAACGATTTGATTGAGC
ACATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGACGAAGCACTGATTACCCAAATCGATGAATATCTT
GATGATACCTTTATGCTGTTTAGCAACTACAGTGTAAATACTCAGGATCTTCAGCGCTGGCAACGCTCAGCGAAACGTTT
ATTTAATGTGTTTACTGAAGAGTGCGCTTTTCTTCAACAGCCAAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References