Detailed information of TA system TA140302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3796893..3797443
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: F4V39_RS19275
• Protein ID: WP_001160975.1
• Coordinates: 3797129..3797443 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: F4V39_RS19270
• Protein ID: WP_000125566.1
• Coordinates: 3796893..3797126 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS19245	3791967..3792254	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
F4V39_RS19250	3792312..3793643	+	1332	WP_001183177.1	MFS transporter	-
F4V39_RS19255	3793751..3794281	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
F4V39_RS19260	3794274..3796136	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
F4V39_RS19265	3796328..3796807	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
F4V39_RS19270	3796893..3797126	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
F4V39_RS19275	3797129..3797443	+	315	WP_001160975.1	CcdB family protein	Toxin
F4V39_RS19280	3797440..3798288	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
F4V39_RS19285	3798295..3798672	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
F4V39_RS19290	3798675..3799496	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
F4V39_RS19295	3799493..3800482	-	990	WP_000241257.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
F4V39_RS19300	3800482..3801768	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T140302 WP_001160975.1 NZ_CP044148:3797129-3797443 [Escherichia coli O157]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T140302 NZ_CP059084:1991166-1991558 [Pantoea ananatis]
ATGGGTTTTACGCTTTATGTTCATAAACGTTTTGACAGAGTGTTTAAAAAAACGCGTCTGACAGATAGCGTCCTGTGTTA
CGCAGCAGGTGACGTTTTTACTGGCGTGTTTGAGGCTGACTTAGGCGGTGGCGTTATTAAAAAGCGCCTTGCTCTTCACC
AAGGCAAACGTGGCGGCGCGCGAACGGTCATCTTCTTCAGACAAGGCACCAACCTGGTTTATTACGATGGATGGGTTAAA
AACTTGTCGAGGAAATACGCCAGGGAAATCGATGAAGATCAGCTGGATACCTACAGGGATGTGGCAAGAATGTTTTTCCG
TGCAAATAGTGAAGTGATGAGCCTGCTCGTCAGAAGCGGAATGGTCAGAGAGGTTAAATGTAATGAAAACTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT140302 WP_000125566.1 NZ_CP044148:3796893-3797126 [Escherichia coli O157]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT140302 NZ_CP059084:1991548-1991901 [Pantoea ananatis]
ATGAAAACTGAACCCGCCGAATTGAGTGAACTACTTGAAGATGCGAAAGCGTTTCACGCGTTAGGACTGGCAACAGATGA
AGAAGTGGAGTCCATAGCAAAGCGCGCAAAAAATCGTGAACTCCGTGCCCGTATTGCATCAGTAAAGCCCATGACAGCAA
CTGAAATCAAAGACGTGCGCATGCGTTGGGGAATGTCGCAATCGCTTGTGGCGCTCTCATTTGGCATGACGGTCGATAGC
GTATCGAAATGGGAGCGAGGTGAAATCACACCGAGCCCACCGGTGATGCGGATGCTACAACTCCTTCGCGATAACGGCCC
TGAACTCTTTACCTCTAAGCATCAAGGTATCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9