Detailed information of TA system TA140297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3547774..3548369
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: F4V39_RS18220
• Protein ID: WP_000239579.1
• Coordinates: 3548019..3548369 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: F4V39_RS18215
• Protein ID: WP_001223210.1
• Coordinates: 3547774..3548025 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS18205	3543439..3547218	+	3780	WP_000060931.1	autotransporter assembly complex protein TamB	-
F4V39_RS18210	3547221..3547562	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
F4V39_RS18215	3547774..3548025	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
F4V39_RS18220	3548019..3548369	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
F4V39_RS18225	3548449..3548979	-	531	WP_000055075.1	inorganic diphosphatase	-
F4V39_RS18230	3549289..3550245	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
F4V39_RS18235	3550385..3551887	+	1503	Protein_3469	sugar ABC transporter ATP-binding protein	-
F4V39_RS18240	3551901..3552923	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T140297 WP_000239579.1 NZ_CP044148:3548019-3548369 [Escherichia coli O157]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T140297 NZ_CP059082:c5247508-5247092 [Halomonas titanicae]
ATGATCGTTCTGGATACCAATGTCGTTTCCGAGGCGATGAAACCCGAGCCTGATCCAGCCGTCAGAGCTTGGCTTAATGA
GCAGTCGGCTGAAACGTTATACCTGTCTAGTGTCACACTTGCAGAGTTGTTATTTGGTATTGCGGCGCTGCCCAACGGGA
AGCGCAAGGACATGTTGAGCGAGACCTTGGGCGGCCTTGTGGAGTTGTTCAGAGGGCGGATTTTGTCCTTTGACGTTGAC
GCCGCTCGCAAGTATGCAGAGCTAGCCGTGACAGCCAGAACCGCCGGGCGTGGTTTTCCTGTGCCGGATGGCTACATTGC
TGCTATTGCCGTATCACAAGGCTATCAAGTCGCCTCCAGAGATATGGCTCCTTTTGAGGCAGCCAATGTGGACGTCATTA
ACCCTTTTGAAGCCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT140297 WP_001223210.1 NZ_CP044148:3547774-3548025 [Escherichia coli O157]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT140297 NZ_CP059082:c5247759-5247505 [Halomonas titanicae]
ATGGGCATGATGACTATTAGAAGCATTCCTGACGAAGTACACAATGCACTAAAAGCGCGAGCCAAGCAGAATCACCGCAG
CGCCGAGGCTGAGGTGCGGGCCATCCTGGAAGAGATCACACGGCCAGAAAGTCGCCTCAAAATGGGTGATGCCCTGGCTG
ATTTGGGTCGCAAGATCGGGTTGACCAATGAAGACGTAGCGGTCTTTGAAAAGGTGCGTGACAAGACGCCTGCTGAACCG
ATGAGGTTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8