Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3450898..3451395 Replicon chromosome
Accession NZ_CP044148
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F4V39_RS17715 Protein ID WP_001173343.1
Coordinates 3451222..3451395 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F4V39_RS17710 Protein ID WP_000398619.1
Coordinates 3450898..3451194 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS17690 3447629..3448348 - 720 WP_000611288.1 two-component system response regulator DcuR -
F4V39_RS17695 3448345..3449976 - 1632 WP_001216477.1 sensor histidine kinase -
F4V39_RS17700 3450157..3450387 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
F4V39_RS17705 3450399..3450671 + 273 WP_000405647.1 N-acetyltransferase -
F4V39_RS17710 3450898..3451194 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F4V39_RS17715 3451222..3451395 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F4V39_RS17720 3451514..3453031 - 1518 WP_001295074.1 lysine--tRNA ligase -
F4V39_RS17725 3453268..3454725 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140296 WP_001173343.1 NZ_CP044148:3451222-3451395 [Escherichia coli O157]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140296 NZ_CP059082:c5151355-5150606 [Halomonas titanicae]
GTGAGTGTGTGGGAAGCCTGTGGCGGCGCGCGAAATATTAAACCTATTTCCGGAACCCTTTATCGACTGGTGGAAAGTCA
GGAGCAAGTCGCAACGCTTAGCTATGTCGACACGTTAGAAGAGCAGGAGATGCTTGAAGCACTGCTTGAAACTGCTAAAC
CGCCTTACCCTGAACAGGAGCGAGACCTGCACTATCTTCTAAAAACCCCTTTTCGGTATCCCCCTCTCAAATGGGGGTCA
CGTTTTGGCCGTGTGTTTGAGCCAAGCATTTTCTACGGTGGTAAGGGCATTGCTACCACTCTCGCTGAATCTGCCTACTA
CCGACTGGTTTTCTGGTACTCCATGGAAGGTGATCCCGTTACTGCCTCGCTGCGCTCAGAGCACACACTCTTTTCTGTAG
ACTATGCCGCTAAACGGGGAGTGAGGCTATACACAGGCTTCTTTGTCGACCACCTTGCGGTACTGAAACATCCAGTTGAT
TACGCCCCGTGTCAGCAATTGGGTAGCGCAATGCGTGGGGCAGGCGTTGAGGCGTTTGAGTATGCCTCCGCAAGAGATAT
TCAGGGTGGTGCGTGCGTGGGCTTGTTTACCCCTGATGCGCTTGCCCAGCATGAGCCAGCCGATATGCGCCCTTGGCTTT
GCGTTACAAGTGCCGCTCAAGTGTCATTTAAACGCAAAGACGCATCAGGCGTTTATACCTACCACCTGGATGATTTTCTT
CACCAAGGCGTGTTGCCTTTCCCTGCTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT140296 WP_000398619.1 NZ_CP044148:3450898-3451194 [Escherichia coli O157]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140296 NZ_CP059082:c5151723-5151352 [Halomonas titanicae]
ATGCCAGCTCTTACTCAAACAAAGCCCACCCCCGAAATGGTGCTAGCTAAAGCAGTTTTGCGTGCTGCTGAGCAATTGGG
TTTATCGCAGTCTGATTTGGCAGAGGTGCTAGGCGTACACCGCACGACTATTACCCAACTTAAAAAAAGCCTGAAGCTTG
ACCCGAACTCTAAGCAGGGTGAACTAGCTCTGCTGCTTATCCGCTTGGCTCGAGCATTATTCGCTCTTACCGGAGGCGAC
CAAACATGGATTCAGCATTTTATGCGAACATCCAATCGTGTTACTGGTGGCATCCCGGCCGAGCAGGTAACACGTTTGGA
GGGGCTGTTTGCGGTACTGAGATTCGTGGATGGTATGCGGGGTAAAGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References