Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2615152..2615783 Replicon chromosome
Accession NZ_CP044148
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag F4V39_RS13750 Protein ID WP_001259385.1
Coordinates 2615508..2615783 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag F4V39_RS13745 Protein ID WP_000593555.1
Coordinates 2615152..2615511 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS13715 2610264..2610737 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
F4V39_RS13720 2610734..2611015 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
F4V39_RS13725 2611092..2612450 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
F4V39_RS13730 2612443..2613951 + 1509 WP_001301773.1 carbohydrate kinase -
F4V39_RS13735 2613941..2614210 + 270 WP_000933060.1 HPr family phosphocarrier protein -
F4V39_RS13740 2614242..2615102 + 861 WP_001131788.1 class II aldolase -
F4V39_RS13745 2615152..2615511 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F4V39_RS13750 2615508..2615783 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
F4V39_RS13755 2615856..2616980 - 1125 WP_001301659.1 ABC transporter permease -
F4V39_RS13760 2616980..2619715 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
F4V39_RS13765 2619712..2620779 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T140293 WP_001259385.1 NZ_CP044148:c2615783-2615508 [Escherichia coli O157]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T140293 NZ_CP059082:c4628387-4628136 [Halomonas titanicae]
ATGAAGACGATCACTTACCGTAAAAGTGCACTAAAGGTGCTGCAGCGAATGCCGCGGCCTGAGTCGAAGCGCATACAAGG
TAAAATCAAGGCCTACGCGGAAGACCCGGAAAGCCAACGCAATAATGTGAAGGCGTTGAAGGGGAGCGATTATATTCGCC
TGCGGGTAGGCGACTGGCGGGTGATCATCGACGAGCGTGGCGATGTGTTGGACATCATTAACATCGGTGCCCGTGGTGCT
ATTTATCAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT140293 WP_000593555.1 NZ_CP044148:c2615511-2615152 [Escherichia coli O157]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT140293 NZ_CP059082:c4628097-4627735 [Halomonas titanicae]
ATGAGTGCACCTCAGATTATTCAAACGCCCACTGGCGAGCGGCTGGTGGTACTGCCCGAGCAGGAGTACCACGCATTGCT
TGCGCGGTTGGAAGACCAGGACGATATCGACGCTGCCAACGCGGTTGTGGCGCGTGGTGAAGAGTCATTTCCCGCAGCGG
TTGTGGATGCGCTGCTGGACGGCGTGGCACCCGTGAAGGTGTACCGTGAATACCGTGGCCTACGCGCAGGTGAACTGGCG
GAAAAAGCAGGGATTAGCCAGGGGTACCTCTCCGAAATAGAGTCAGGAAAAAAGAGCGGCTCGTTGAGTGTGCTGACCCG
CATTGCGGCTGCGTTGGATGTGGAGTTGAGAGATATTACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References