Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2572553..2573353 Replicon chromosome
Accession NZ_CP044148
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag F4V39_RS13500 Protein ID WP_000342448.1
Coordinates 2572553..2573080 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag F4V39_RS13505 Protein ID WP_001277108.1
Coordinates 2573087..2573353 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS13475 2567628..2568395 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
F4V39_RS13480 2568392..2569669 - 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
F4V39_RS13485 2569666..2570592 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
F4V39_RS13490 2570640..2571749 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
F4V39_RS13495 2572173..2572556 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
F4V39_RS13500 2572553..2573080 - 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
F4V39_RS13505 2573087..2573353 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
F4V39_RS13510 2573503..2574606 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
F4V39_RS13515 2574877..2575731 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
F4V39_RS13520 2575976..2577034 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
F4V39_RS13525 2577027..2577695 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T140292 WP_000342448.1 NZ_CP044148:c2573080-2572553 [Escherichia coli O157]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T140292 NZ_CP059082:c2798102-2797806 [Halomonas titanicae]
ATGATCGAGTTGATTAAAACCGATGTTTTCGATAGATGGCTACGCGAGCTTCGCGATATCCGTGCCCGCGCCAAGGTTGA
AGCCCGCATCCGACGTTTGAGTCTGGGCAACCCTGGAGATGTGAAGCCGGTCGGTAAAGGCGTTTCTGAACTGCGAATTC
CTTATGGGCCAGGCTACCGCGTCTATTATATTACCAAAGGGCCAGTAATCGTGGTTCTGCTGTGCGGTGGTGATAAAGCC
ACCCAATCCCACGATATCGAACTAGCCAAAGCCATTGCCGCGCAATGGAAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT140292 WP_001277108.1 NZ_CP044148:c2573353-2573087 [Escherichia coli O157]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT140292 NZ_CP059082:c2797801-2797487 [Halomonas titanicae]
ATGACCGTTCCTAATGTCACTTTCAGTCACTACGATACTGCTGATTACCTTCAAACTGAGGAGGATATTGCGGCCTATCT
TGATGCAGTAATGGAAGAAAATGATCCTGCTCTTCTTGCCGCCGCACTTGGTGATATCGCCCGTGCCCGCAACATGAGCC
AACTCGCCAAAGACGTAGGCATGAGCCGTGAAGGGCTTTACAAAGCCCTATCGGGGGAAGGAAACCCGGCTTTTGCGACC
ATTAGCAAAGTAGCCAACTCCTTGGGGTTGCGCCTATCTATTAAACCGCTATCGCGGCCAGAAGCGGGCAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References