Detailed information of TA system TA140291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 2547213..2549541
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: F4V39_RS13375
• Protein ID: WP_000532146.1
• Coordinates: 2548567..2549541 (-)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: F4V39_RS13370
• Protein ID: WP_001303704.1
• Coordinates: 2547213..2548508 (-)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS13355	2542820..2545006	-	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-
F4V39_RS29560	2545164..2545205	-	42	WP_211180509.1	protein YhgO	-
F4V39_RS13360	2545279..2546382	-	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
F4V39_RS13365	2546574..2547167	+	594	WP_001002544.1	NAAT family transporter YhgN	-
F4V39_RS13370	2547213..2548508	-	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
F4V39_RS13375	2548567..2549541	-	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
F4V39_RS13380	2549545..2549946	-	402	WP_000502498.1	ATP-dependent DNA helicase	-
F4V39_RS13385	2549931..2550539	-	609	WP_000410808.1	ATP-dependent DNA helicase	-
F4V39_RS13390	2550679..2552019	-	1341	WP_000210111.1	gluconate transporter	-
F4V39_RS13395	2552023..2552550	-	528	WP_000108330.1	gluconokinase	-
F4V39_RS13400	2552689..2553684	-	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T140291 WP_000532146.1 NZ_CP044148:c2549541-2548567 [Escherichia coli O157]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T140291 NZ_CP059082:c2797801-2797487 [Halomonas titanicae]
ATGACCGTTCCTAATGTCACTTTCAGTCACTACGATACTGCTGATTACCTTCAAACTGAGGAGGATATTGCGGCCTATCT
TGATGCAGTAATGGAAGAAAATGATCCTGCTCTTCTTGCCGCCGCACTTGGTGATATCGCCCGTGCCCGCAACATGAGCC
AACTCGCCAAAGACGTAGGCATGAGCCGTGAAGGGCTTTACAAAGCCCTATCGGGGGAAGGAAACCCGGCTTTTGCGACC
ATTAGCAAAGTAGCCAACTCCTTGGGGTTGCGCCTATCTATTAAACCGCTATCGCGGCCAGAAGCGGGCAGCTAA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT140291 WP_001303704.1 NZ_CP044148:c2548508-2547213 [Escherichia coli O157]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT140291 NZ_CP059082:c2798102-2797806 [Halomonas titanicae]
ATGATCGAGTTGATTAAAACCGATGTTTTCGATAGATGGCTACGCGAGCTTCGCGATATCCGTGCCCGCGCCAAGGTTGA
AGCCCGCATCCGACGTTTGAGTCTGGGCAACCCTGGAGATGTGAAGCCGGTCGGTAAAGGCGTTTCTGAACTGCGAATTC
CTTATGGGCCAGGCTACCGCGTCTATTATATTACCAAAGGGCCAGTAATCGTGGTTCTGCTGTGCGGTGGTGATAAAGCC
ACCCAATCCCACGATATCGAACTAGCCAAAGCCATTGCCGCGCAATGGAAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8