Detailed information of TA system TA140287

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2035588..2036242
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: F4V39_RS10735
• Protein ID: WP_000244781.1
• Coordinates: 2035588..2035995 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: F4V39_RS10740
• Protein ID: WP_000354046.1
• Coordinates: 2035976..2036242 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS10715	2031545..2033278	-	1734	WP_000813186.1	single-stranded-DNA-specific exonuclease RecJ	-
F4V39_RS10720	2033284..2033994	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
F4V39_RS10725	2034019..2034915	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
F4V39_RS10730	2035027..2035548	+	522	WP_001055874.1	flavodoxin FldB	-
F4V39_RS10735	2035588..2035995	-	408	WP_000244781.1	protein YgfX	Toxin
F4V39_RS10740	2035976..2036242	-	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
F4V39_RS10745	2036485..2037465	+	981	WP_000886053.1	tRNA-modifying protein YgfZ	-
F4V39_RS10750	2037542..2038201	-	660	WP_000250274.1	hemolysin III family protein	-
F4V39_RS10755	2038365..2038676	-	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
F4V39_RS10760	2038721..2040154	+	1434	WP_001310226.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T140287 WP_000244781.1 NZ_CP044148:c2035995-2035588 [Escherichia coli O157]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T140287 NZ_CP059082:c1332618-1332154 [Halomonas titanicae]
ATGAGCGAGGTCACCGCCTATCGGCTGGTTAAGCGTAAGTTTCAGGAGACAGCGTTTGATGGCGAAGGCGCACGGCTTTA
CGGTGGGCGCTGGAACAGCCCAGGGAATGCCTGTGTGTATGTGGCAAGCTACGAGTCGCTGGCACTACTGGAAATTATGG
TGAATCTGGAAAGCTATCGGCTGCTCAATGCGTATGCGCTGCTGCGCCTGACGCTACCAGCCGACAGTATTCTAAGCATT
GGCGCAGAAGATCTACCAGAAAACTGGCAGGAAGCCCCAGCGCCCGCAGAGACCGCTGAACTTGGCGATGGCTGGCTGGC
CTCAGGCCAAAGTCTGGCACTGGCAGTGCCAAGCGTGGTCGTGCCGAGAGAGCTCAACTACATGCTCAACCCGGCACACC
CCTTATTCGATCAAATCGTCGCGACAGCCGAGGCACTGCCATTTCAACCCGATCCAAGACTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT140287 WP_000354046.1 NZ_CP044148:c2036242-2035976 [Escherichia coli O157]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT140287 NZ_CP059082:c1333064-1332615 [Halomonas titanicae]
ATGGATATCCACTTCACTCAATACCAGCCACCCAAAGCTTTGGCGTCCGGTTTCTGGCAACAAATTGGCTTGCCAGCCAG
AGGCCGCAAGTTGCATGAGCTGTTGCATGAAGGGGTGGAGTATCGTGTTTATCCCCAGATTGCCAAGGTATCAGGTATTG
AGCAGAAGGCACTGGCGCGCTACGTGGATATTCCCTCTGCTACGCTTAGCCGTCGCGCCAAATCGGGCCGTTTCAAAATG
GCAGAGAGTGACCGGCTGTATCGCTTTGCTGAGCTTTTCAAGGCGGCACTGGATCTGTTCGAGGGGGACTCAGAGGGCGC
CCGCGCCTGGCTACTCAAACCCAATGCCGGGCTAGGCGGTCGTCGCCCTGTCGAAATGAGCGCCACATCGGCAGAGTATC
AAACGGTGTTGAATCTGATCGGGCGGCTGGAGCATGGTGTCTCGGTATGA

Structures

Toxin

• AlphaFold DB: S1PAM6

Antitoxin

• PDB: 6B58
• PDB: 1X6I
• PDB: 1X6J
• PDB: 6C12
• AlphaFold DB: A0A7U9QD57