Detailed information of TA system TA140277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 353853..354324
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: F4V39_RS01830
• Protein ID: WP_001303511.1
• Coordinates: 354046..354324 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: F4V39_RS01825
• Protein ID: WP_001302048.1
• Coordinates: 353853..354044 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS01785	349060..349644	-	585	WP_000206793.1	DUF551 domain-containing protein	-
F4V39_RS01790	349701..350096	-	396	WP_001118159.1	DUF977 family protein	-
F4V39_RS01795	350112..350881	-	770	Protein_345	DUF1627 domain-containing protein	-
F4V39_RS01800	350907..351647	-	741	WP_000788938.1	ATP-binding protein	-
F4V39_RS01805	351654..352616	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
F4V39_RS01810	352639..353064	-	426	WP_000693943.1	Rha family transcriptional regulator	-
F4V39_RS01815	353061..353363	-	303	WP_000172738.1	transcriptional regulator	-
F4V39_RS01820	353461..353832	+	372	WP_001169686.1	hypothetical protein	-
F4V39_RS01825	353853..354044	+	192	WP_001302048.1	hypothetical protein	Antitoxin
F4V39_RS01830	354046..354324	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V39_RS01835	354676..354975	+	300	WP_001240334.1	hypothetical protein	-
F4V39_RS01840	355047..355265	+	219	WP_001171930.1	hypothetical protein	-
F4V39_RS01850	355834..356022	+	189	WP_000449192.1	cell division inhibition protein DicB	-
F4V39_RS01855	356019..356210	+	192	WP_001090200.1	DUF1482 family protein	-
F4V39_RS01860	356303..358774	+	2472	WP_000048458.1	exonuclease	-
F4V39_RS01865	358847..359098	+	252	WP_000005552.1	excisionase family protein	-
F4V39_RS01870	359133..359317	+	185	Protein_359	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	264451..369203	104752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T140277 WP_001303511.1 NZ_CP044148:354046-354324 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T140277 NZ_CP059078:c1192781-1192518 [Acinetobacter nosocomialis]
ATGAAACGGAAGACTAGTTTTACTCCAATAGCTTGGGAGGATTACGTGTATTGGCAAAACCAAGACAAAAAGACTCTTAA
GCGCATCAATACATTAATCAAAGAGTGTCTTCGTACCCCCTTTGATGGTATTGGCAAGCCTGAACCATTACTCGGCGATT
TAGCAGGTTTGTGGAGCAGACGAATAGATGAAAAAAATAGATTAGTCTATGAAGTAACTGATGTGGCGATAACTGTCATT
TCTTGTCGTTATCACTACGAATAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT140277 WP_001302048.1 NZ_CP044148:353853-354044 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT140277 NZ_CP059078:c1193028-1192765 [Acinetobacter nosocomialis]
ATGCAAATTTTCACTTATACCGATGCTCGCAATAATCTGAAAACGGTTTTAGATCGTGTCATTGATGATTCAGATGTTGC
AGTAATTACGAGAAAAGAAGGTCAACATGCGGTTGTAATGGGACAAGACTATTACAACAGTATAATGGAAACATTGCACC
TCATGTCTTCTCCAAGTAATGCAGCACACCTAGCAAAATCAATTGCAGAATTACGGGCATCTAAAGCAATCGAAAGAGAA
CTTTTAGATGAAACGGAAGACTAG

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0