140277

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 353853..354324 Replicon chromosome
Accession NZ_CP044148
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag F4V39_RS01830 Protein ID WP_001303511.1
Coordinates 354046..354324 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag F4V39_RS01825 Protein ID WP_001302048.1
Coordinates 353853..354044 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS01785 349060..349644 - 585 WP_000206793.1 DUF551 domain-containing protein -
F4V39_RS01790 349701..350096 - 396 WP_001118159.1 DUF977 family protein -
F4V39_RS01795 350112..350881 - 770 Protein_345 DUF1627 domain-containing protein -
F4V39_RS01800 350907..351647 - 741 WP_000788938.1 ATP-binding protein -
F4V39_RS01805 351654..352616 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
F4V39_RS01810 352639..353064 - 426 WP_000693943.1 Rha family transcriptional regulator -
F4V39_RS01815 353061..353363 - 303 WP_000172738.1 transcriptional regulator -
F4V39_RS01820 353461..353832 + 372 WP_001169686.1 hypothetical protein -
F4V39_RS01825 353853..354044 + 192 WP_001302048.1 hypothetical protein Antitoxin
F4V39_RS01830 354046..354324 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F4V39_RS01835 354676..354975 + 300 WP_001240334.1 hypothetical protein -
F4V39_RS01840 355047..355265 + 219 WP_001171930.1 hypothetical protein -
F4V39_RS01850 355834..356022 + 189 WP_000449192.1 cell division inhibition protein DicB -
F4V39_RS01855 356019..356210 + 192 WP_001090200.1 DUF1482 family protein -
F4V39_RS01860 356303..358774 + 2472 WP_000048458.1 exonuclease -
F4V39_RS01865 358847..359098 + 252 WP_000005552.1 excisionase family protein -
F4V39_RS01870 359133..359317 + 185 Protein_359 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 264451..369203 104752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T140277 WP_001303511.1 NZ_CP044148:354046-354324 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T140277 NZ_CP059078:c1192781-1192518 [Acinetobacter nosocomialis]
ATGAAACGGAAGACTAGTTTTACTCCAATAGCTTGGGAGGATTACGTGTATTGGCAAAACCAAGACAAAAAGACTCTTAA
GCGCATCAATACATTAATCAAAGAGTGTCTTCGTACCCCCTTTGATGGTATTGGCAAGCCTGAACCATTACTCGGCGATT
TAGCAGGTTTGTGGAGCAGACGAATAGATGAAAAAAATAGATTAGTCTATGAAGTAACTGATGTGGCGATAACTGTCATT
TCTTGTCGTTATCACTACGAATAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT140277 WP_001302048.1 NZ_CP044148:353853-354044 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT140277 NZ_CP059078:c1193028-1192765 [Acinetobacter nosocomialis]
ATGCAAATTTTCACTTATACCGATGCTCGCAATAATCTGAAAACGGTTTTAGATCGTGTCATTGATGATTCAGATGTTGC
AGTAATTACGAGAAAAGAAGGTCAACATGCGGTTGTAATGGGACAAGACTATTACAACAGTATAATGGAAACATTGCACC
TCATGTCTTCTCCAAGTAATGCAGCACACCTAGCAAAATCAATTGCAGAATTACGGGCATCTAAAGCAATCGAAAGAGAA
CTTTTAGATGAAACGGAAGACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
AlphaFold DB A0A829CTP0

References